摘要
黑曲霉（Ａｓｐｅｒｇｉｌｕｓｎｉｇｅｒ）在工业生产和科学研究中占有重要地位，因为许多黑曲霉菌株能产生有机酸和酶制剂，并能分泌大量蛋白质到细胞外［１，６］．三磷酸甘油醛脱氢酶（Ｇｌｙｃｅｒａｌｄｅｈｙｄｅ３ｐｈｏｓｐｈａｔｅｄｅｈｙｄｒｏｇｅｎａ...
Fragments produced by Eco RI digestion of the chromosomal DNA of the glucoamylase producing strain aspergillus niger SG were inserted into the Eco RI site of the vector λ ZAPⅡ,then the recombinant DNA molecules were packaged in vitro to form the genomic library.Through screening the genomic library by hybridization a positive clone was obtained and primarily characterized.The results showed that the cloned 10 kb Eco RI fragment contained the complete A.niger glyceraldehyde 3 phosphate dehydrogenase gene with 2.4 kb in size.
出处
《应用与环境生物学报》
CAS
CSCD
1998年第1期88-91,共4页
Chinese Journal of Applied and Environmental Biology
基金
英国文化委员会的部分资助
关键词
三磷酸甘油醛
脱氢酶
基因
黑曲霉
基因组文库
glyceraldehyde 3 phosphate dehydrogenase gene
Aspergillus niger
genomic library
gene cloning
Southern hybridization
