玉米细胞质HSC70:一种钙调素结合蛋白

PURIFICATION OF MAIZE CYTOSOLIC 70 kD STRESS PROTEIN:A CALMODULINBINDING PROTEIN IN PLANTS

玉米细胞质ＨＳＣ７０：一种钙调素结合蛋白孙旭彤２周人纲１汤文强１孙大业１（河北师范大学生物系石家庄０５００１６）２（河北省农业科学院农业物理生理生化研究所石家庄０５００５１）关键词玉米热激蛋白７０，钙调素结合蛋白ＰＵＲＩＦＩＣＡＴＩＯＮＯＦＭＡＩ...

The maize cytosolic 70 kD stress protein (HSC70) has been purified by a twostep procedure employing affinity chromatography on ATPagarose followed by DEAE52 ionexchange chromatography. Using a biotinylated cauliflower calmodulin (CaM) geloverlay technique in the presence of 1 mmol/L Ca2+, the HSC70 could bind to CaM. No band was shown on sodium dodecyl sulfatepolyacrylamide gel overlayed with biotinylated cauliflower CaM when 1 mmol/L Ca2+was replaced by 5 mmol/L EGTA. It indicated that the binding of HSC70 to CaM was dependent on Ca2+. The purified HSC70 inhibited the activity of CaMdependent NADK and the degree of inhibition increased with augmentation of the HSC70,which appeared to be typically characteristic to CaMbinding protein.