齿兰环斑病毒外壳蛋白基因克隆及序列分析

MOLECULAR CLONING AND SEQUENCING OF COAT PROTEIN GENE OF ODONTOGLOSSUM RINGSPOT VIRUS

齿兰环斑病毒外壳蛋白基因克隆及序列分析刘志昕潘俊松邵寒霜郑学勤（中国热带农业科学院热带作物生物技术国家重点实验室，海南儋州５７１７３７）关键词齿兰环斑病毒，外壳蛋白基因，序列分析兰花受病毒危害相当严重，齿兰环斑病毒（Ｏｄｏｎｔｏｇｌｏｓｕｍｒｉｎｇｓ...

Odontoglossum ringspot virus(ORSV)was isolated from the Cattleya orchid cultivated in Hainan.The coat protein(CP)gene of the ORSV was amplified from the extracted viral RNA by using reverse transcription-polymerase chain reaction(RT-PCR)method,and cloned into plasmid BluescriptⅡ SK by inserting into EcoRV site.Its complete uncleotide sequence has been determind and compared with the ORSV isolated from Cymbidium orchid of Japan.They share 99.2% and 98.1% identity at the sequence levels of nucleotide and deduced amino acid respectively.