我国丙型肝炎病毒囊膜蛋白E_2高变区1的序列特征

Sequence Characterization of Hypervariable Region 1 of the Putative Envelope Protein 2 in Chinese HCV Isolates

对２３例国内献血员、血透析及肝炎病人血清用反转录巢式ＰＣＲ技术扩增了ＨＣＶＲＮＡ囊膜蛋白２基因的ｃＤＮＡ片段，并进行了序列测定。结果表明２３例病人ＨＣＶＥ２／ＮＳ１Ｎ末端的核苷酸及氨基酸序列呈现多样性，高变区１（ＨＶＲ１）位于核苷酸第１４５９～１５５９位，氨基酸第３８４～４１０位；我国ＨＣＶ株ＨＶＲ１２７个氨基酸中有１５个位置氨基酸相对稳定，氨基酸组成与分布均与Ｓｅｋｉｙａ报道的１６６个ＨＣＶ株的不同。结果提示，研究我国ＨＣＶ株ＨＶＲ１的序列特征有助于ＨＣＶ的流行病学研究，对研制适用于我国的抗体诊断试剂盒及进行疫苗研究均有重要的意义。

Twenty three serum samples from blood donor, hemodialysis and hepatitis patients were collected. Using reverse transcription nested polymerase chain reaction method (RT nested PCR),the cDNA fragments of the putative envelope protein 2 (E 2/NS 1) of HCV RNA were amplified and then sequenced. Nucleotied and amino acid sequence analysis showed marked heterogeneity among isolates from 23 patients at the N terminus of E 2/NS 1.The extended sequence analysis comfirmed that the hypervariable region one (HVR 1) is located at the positions 1459～1559 in nucleotide sequence,and 384～410 in amino acid sequence. It was found that 15 amino acids of 27 amino acids of HVR 1 in Chinese HCV isolates were stable, and the composition and distribution of amino acid of HVR 1 were much different from 166 isolates reported by Sekiya. It is suggested that studying the characterization of HVR 1 HCV Chinese isolates would be helpful for investigating geographical distribution of HCV, and researching diagnosis method and vaccine in China.