原位分子杂交法检测恙螨体内肾综合征出血热病毒核酸的研究

Detection of Hemorrhagic Fever with Renal Syndrome Virus RNA in Chigger Mite by in situ Hybridization

为探讨肾综合征出血热病毒（ＨＦＲＳＶ）在恙螨体内的分布和定位，采用原位分子杂交技术检测恙螨体内ＨＦＲＳＶＲＮＡ。结果发现：原位分子杂交技术的检出阳性率较ＩＦＡＴ高；ＨＦＲＳＶ阳性信号颗粒呈弥散分布，多见于恙螨幼虫和若虫的腹部组织细胞内，该部位是恙螨的卵巢细胞、中肠及支囊上皮细胞。前体组织细胞内少见；个别幼虫和若虫的前足和中足细胞内亦可见到ＨＦＲＳＶＲＮＡ阳性信号。在原位分子杂交中，若虫组织细胞内的ＨＦＲＳＶＲＮＡ阳性信号较幼虫密集而且量多。

In order to study the distribution and position of HFRSV in chigger mite, in situ hybridization was used for detection of hemorrhagic fever with renal syndrome virus (HFRSV) RNA.The results showed that positive rate of HFRSV by in situ hybridization was higher than that by IFAT. Positive signals were observed mostly in belly cells of larva and nymph. The positions were probably ovary cells and midgut. A few positive signals were seen in foreleg and middle leg cells also. The positive signals of HFRSV in nymph were much more than in larva. It demonstrated that HFRSV can be transmitted by trans stadial and proliferate in mites.