我国真菌传线状小麦花叶病毒病病原初步鉴定为小麦黄花叶病毒(WYMV)

Identification of Chinese Fungal transmitted Filamentous Wheat Mosaic Virus by RT PCR and SSCP

由禾谷多粘菌（Ｐｏｌｙｍｙｘａｇｒａｍｉｎｉｓ）传播的线状小麦花叶病毒有两种，一种是加拿大首先报道的小麦梭条斑花叶病毒（ＷＳＳＭＶ），另一种是日本报道的小麦黄花叶病毒（ＷＹＭＶ）。这两种病毒粒子形态以及血清学性质非常相似，但核酸序列存在一定差异。经反转录聚合酶链反应（ＲＴＰＣＲ）和单链构象多态性分析（ＳＳＣＰ），明确我国广泛发生的禾谷多粘菌传线状小麦花叶病毒都是小麦黄花叶病毒，但供试的８个分离物ＲＮＡ１和ＲＮＡ２序列存在差异。

There are two filamentous wheat mosaic viruses transimitted by the plasmodiophoraceous fungus Polymyxa graminis :one is wheat spindle streak mosaic virus (WSSMV)first reported in Canada and the other is wheat yellow mosaic virus (WYMV) first reported in Japan .These viruses are very similar in particle morphology,biological and serological properties, but different in genomic sequences. The results of RT-PCR and single strand conformation polymorphism analysis (SSCP) indicated that the fungal transmitted filamentous wheat mosaic viral pathogen occurred in successive crops at different sites in China is wheat yellow mosaic virus. SSCP analysis with the fragments amplified using several primer sets all gave different patterns ,indicating the presence of sequence variations among eight isolates collected at different sites in China.