摘要
在人纤维蛋白原制备工艺中增加S/D处理灭活病毒步骤,TNBP和Tween80终浓度分别为0.3%和1%,在25℃处理6小时能有效灭活指示病毒VSV(〉3.75Log)、Sindbis(〉4.46Log)、HIV(〉3.67Log)。
Virus inactivation with S/D treatment was incorporated into the preparation scheme of human fibrinogen. The final concentration of TNBP and Tween80 in the solution was 0.3% and 1% respectively. The 6 hour treatment at 25℃ could sterilize the marker viruses effectively e.g VSV (>3.75Log),Sindbis(>4.46Log),HIV(>3.67Log). The viruses were not detected in the three blind passage.
出处
《微生物学免疫学进展》
1998年第1期46-50,共5页
Progress In Microbiology and Immunology
关键词
人纤维蛋白原
S/D处理
病毒灭活
验证
Human fibrinogen S/D treatment Validation of virus inactivation