海藻中清除氧自由基的物质

THE SUBSTANCES SCAVENGING OXYGEN FREE RADICALS IN MARINE ALGAE

新鲜海藻的提取液含有超氧物歧化酶（SOD）活性物质，能清除超氧自由基（O2-）。海藻的SOD活性通常为60—280Ug-1FW，而在孔石莼（Ulvapertusa）、江蓠（Gracilariaverrucosa）和凤尾菜（G．eucheumoices）中活性较高，约为300Ug-1FW。一般来说，海藻的SOD活性和稳定性为：绿藻＞红藻＞褐藻。绿藻的SOD以CuZn-型为主，而蓝藻的SOD以Fe-型为主。以江蓠琼枝（Eucheumagelatinae）提取液作PAGE并SOD活性染色时，除了观察到SOD同工酶带之外，还发现在前沿指示剂附近有一区域，此区域与高效自由基清除剂SPD（Superphycodismutas）的电泳行为和对氮蓝四唑（NBT）负染色的抑制相同，可能两者为同一种物质。

Some substances with activity of superoxide dismutase (SOD) extracted from fresh algae can scavenge superoxide radical (O). The activity of SOD in algae is usually 60 - 280 U g-1FW, but that in Ulva pertusa, Gracilaria verrucosa and G. eucheumoides is higher (about 300 U g-1FW). In general, the activity and stability of SOD in algae are in order: green algae>red algae>brown algae. The main types of SOD in green algae are CuZn-SOD, but in blue algae, Fe-SOD. The result of PAGE and SOD active staining of the extracts from G. verrucosa and Eucheuma gelatinae showed that besides some bands with SOD activity, there was also one region near the front of indicator which had similar electrophoreses behavior and similar inhibition of photochemical reduction by NBT, as seen on the band of SPD (Superphycodismutase) which is a highly effective scavenger of free radicals, showing that they may be the same substance.