摘要
目的检测H9N2禽流感病毒血凝素（hemagglutinin．HA）和神经氨酸酶（neuraminidase，NA）DNA疫苗保护小鼠抵抗致死量同源病毒感染的能力。方法通过小鼠肺对肺传代，建立禽流感病毒A／chicken／Jiangsu／07／2002（H9N2）小鼠适应株。同时，构建病毒HA和NADNA疫苗，以不同剂量电击法免疫小鼠1或2次，在初次免疫后4周或加强免疫后1周用致死量（40 LD50）鼠适应型病毒攻击小鼠。通过测定小鼠血清抗体滴度、小鼠存活率和肺部病毒滴度来评价疫苗的效果。结果HA或NA DNA10μg免疫1次或3μg免疫2次均可保护小鼠抵抗致死量H9N2病毒的感染。结论低剂量HA或NADNA可为抗H9N2禽流感病毒感染提供有效的免疫保护。
Objective To test the protective ability of avian H9N2 virus hemagglutinin （HA） and neuraminidase （NA） DNA against lethal virus challenge in mice model. Methods Viral lung-to-lung passages in mice were used to obtain a mouse-adapted strain for avian H9N2 isolate A/chicken/Jiangsu/07/2002. DNA vaccines expressing H9N2 HA and NA, respectively, were constructed to immunize mice by electroporation. Mice were immunized once or twice （ at a 3-week interval） with various dosages of DNA vaccine and were then challenged with a lethal dose （ 40 LD50 ） of mouse-adapted virus. The effectiveness of the two DNAs was evaluated by serum specific antibody titers of mice, survival rates and lung virus titers. Results Immunization once with HA or NA DNA at a dosage of 10 μg or twice at a dosage of 3 μg provided protection for mice against lethal virus challenge. Conclusion Either H9N2 virus HA or NA DNA can be effective in defending avian influenza virus infection.
出处
《国际生物制品学杂志》
CAS
2009年第2期57-63,共7页
International Journal of Biologicals
基金
国家自然科学基金资助项目（30170043）
