刺参糖胺聚糖抗仙台病毒作用机制的探讨

Studyon Anti-Sendai Virus Mechanism of Stichopus Japomicus Slelenka Glycosaminoglycans

目的:探讨刺参糖胺聚糖的抗仙台病毒(Sendai virus,SV)作用及其作用机制。方法:选用不同浓度刺参糖胺聚糖作用于仙台病毒感染BHK21细胞的多个环节,倒置显微镜观察病毒致细胞病变效应,MTT法检测细胞活性;同时以SV滴鼻感染小鼠,观察刺参糖胺聚糖对感染病毒小鼠血凝抑制抗体产生的影响。结果:刺参糖胺聚糖浓度大于3.2mg/ml时表现出细胞毒性作用。浓度在0.25～0.2mg/ml范围时,刺参糖胺聚糖作用病毒吸附组和病毒复制组能明显抑制细胞病变,MTT结果也表明该两组显示较好的细胞活性,且具有一定的量效关系,无细胞毒性作用;但SJ-GAG直接作用病毒组、预处理细胞组以及抑制病毒穿入组无明显抗病毒作用。动物实验表明刺参糖胺聚糖可促进小鼠血凝抑制抗体的产生。结论:刺参糖胺聚糖的抗仙台病毒作用主要通过抑制病毒对靶细胞的吸附以及抑制病毒复制而实现,并能促进小鼠对病毒感染的免疫应答。

Objective： To study the anti-Sendai virus effect of Stichopus Japomicus Slelenka Glycosaminoglycans and its mechanism. Methods： The BHK21 cells were infected with SV, and the S J-GAG was used at different steps of SV replication cycle. The cytopathic effect （CPE） was observed by inverted microscope. The cytoactive was tested by MTT. In vivo the mice were infected with SV by nose, then the influence on hemagglutination inhibition antibodies of S J-GAG was tested. Results： When the concentration of SJ-GAG was above 3.2mg/ml, it showed cytotoxicity. When the concentration was 0.25-0.2mg/ml, SJ-GAG could inhibit CPE in virus adhension group and virus replication group. The result of MTT suggested that the two groups had better cytoactive, besides the effect dependence on the concentration without cytotoxicity, but there was no obvious antiviral effect in S J-GAG direct-acting group, pretreatment group and viral penetration group. The result of animal test suggested that S J-GAG could accelerate the production of hemagglutination inhibitor antibodies in mice. Conclusion： S J-GAG has antiviral effect by inhibiting the adhension and replication of SV. It can advance the immunity response to virus infection in mice.