共轭亚油酸对HepG2细胞脂质沉积的影响

The Effect of Conjugated Linoleic Acid (CLA) on Lipid Deposition in HepG2 Cells

目的:研究共轭亚油酸(conjugated linoleic acid,CLA)对HepG2细胞脂肪沉积的影响,以探讨其对肝脏脂肪沉积的可能机制。方法:体外培养HepG2细胞,不同浓度CLA(0、10、50、100μmol.l-1)作用24小时后,100nmol.l-1胰岛素作用1小时。以比色法测定培养液中游离脂肪酸的含量,油红O染色观察细胞脂肪沉积,Western-blot检测Akt和P-Akt蛋白表达。结果:随着CLA浓度增加,培养液中的游离脂肪酸含量显著降低,细胞中红染脂滴增多,Western-blot结果发现,Akt蛋白的磷酸化水平增高。结论:CLA可以增加细胞内脂肪合成和细胞中Akt蛋白的磷酸化水平。

Objective： To investigate the effect of conjugated linoleic acid （CLA） on lipid deposition in HepG2 cells and its possi- ble mechanisms. Methods： HepG2 cells were incubated with different concentration of CLA （0, 10, 50, 100 μmol.l^-1） for 24 hours, then in the presence of insulin （100 nmol.l^-1） for one hour. Free fatty acid content was determined using colorimetric method. Oil red O stain was used to observe lipid deposition. Western-blot was used to detect Akt and P-Akt protein expression. Results： Along with the increasing of CLA concentration, the free fatty acid in the medium was decreased significantly, and the red lipid droplets were increasing in cells. Results of the Western-blot showed Akt protein phosphorylation level was also increased. Conclusions： CLA can increase fat synthesis and phosphorylation level of Akt protein in the cells.