体外胶质瘤细胞原代培养对不同化疗药物敏感性初步研究

A Preliminary Study on Ex-vitro Drug Sensitivity Resistance Assay of Primary Culture Glioma cells

目的:观察7种抗肿瘤药物对体外原代培养的胶质瘤细胞的作用,为临床化疗方案的设计提供参考。方法:32例术后病理证实为胶质瘤的新鲜标本取材,制备肿瘤单细胞悬液进行体外原代培养,与肿瘤药DDP,VM-26,VP-16,BCNU,VCR,PCB,Taxol的临床血浆峰值浓度(PPC)作用72小时,MTT法检测不同肿瘤个体对不同化疗药物的敏感和耐药情况。结果:成功进行了32例临床标本的原代培养及药敏试验,其PPC下的抑制率(IR%)>50%者,DDP有14例;VCR有5例;VM-26有7例;VP-16有9例;PCB有3例;BCNU有2例;Taxol有1例;其敏感性依次为:DDP>VP-16>VM-26>VCR>PBZ>BCNU>Taxol。结论:7种常用的抗肿瘤药物均有耐药的情况,进行化疗药物的敏感测定可以避免耐药药物的使用,为提高临床化疗效果提供指导。

Objective： To carry out chemosensitivity experiment with the MTT assay aiming at providing theory guidance for clinical chemotherapy regimen Methods： 32 fresh specimen which were proved to be neurogliocytoma were used to produce monoplast suspension and carry out vitro primary culture, then seven antineoplastic drugs were added with peak plasma concentration to the primary culture glioma cells for 72 hours. Then the MTT colorimetric assay was employed to measure the survival tumor cell, to get the optical density and the inhibition ratio, and judge the sensitiveness and resistance to guide the clinical individual chemotherapy. ResultsL 32 clinical gliomas specimen were subject to primary culture and susceptibility test. The rank of sensitivity from high to low is： DDP〉 VP-16〉 VM-26〉VCR 〉PCB〉BCNU〉Taxol. Conclusions： Every one of 7 commonly used drugs has drug resistance. Chemosensitivity assay can avoid using the resistant drugs and provide guidance to improve the clinical chemotherapy effect.