苯丙氨酸、蔗糖和甘露醇对杂种红豆杉细胞的生长及形成紫杉醇、巴卡亭III和10-去乙酰基巴卡亭III的影响

EFFECTS OF PHENYLALANINE, SUCROSE AND MANNITOL ON THE GROWTH AND PRODUCTION OF TAXOL, BACCATIN III AND 10DEACETYLBACCATIN III IN SUSPENSION CELLS OF TAXUS MEDIA

研究了苯丙氨酸、蔗糖和甘露醇对杂种红豆杉悬浮细胞的生长及形成紫杉醇、巴卡亭ＩＩ和１０去乙酰基巴卡亭ＩＩ的影响。结果表明，培养基中添加１０ｍｍｏｌ·Ｌ－１或２０ｍｍｏｌ·Ｌ－１苯丙氨酸和在培养２８ｄ时同时补加７３０ｍｍｏｌ·Ｌ－１蔗糖和１３７３ｍｍｏｌ·Ｌ－１甘露醇能显著地促进细胞的生长和这３种紫杉烷的形成。同对照相比，有苯丙氨酸又补加蔗糖和甘露醇的细胞生物量增加了０６～０８倍，紫杉醇的产量增加了９～１０倍，巴卡亭ＩＩ的产量增加了２５～３０倍，１０去乙酰基巴卡亭ＩＩ的产量增加了７倍。在培养２８ｄ时补加７３０ｍｍｏｌ·Ｌ－１蔗糖能显著地促进细胞的生长，但对细胞中这３种紫杉烷的含量没有显著的影响。

The effects of phenylalanine, sucrose and mannitol on the cell growth and the production of taxol, baccatin III and 10deacetylbaccatin III in the suspension cells of Taxus media were studied. The results showed that phenylalanine 10 mmol·L-1 or 20 mmol·L-1 initially added into the medium, and sucrose 730 mmol·L-1 and mannitol 1733 mmol·L-1 added into the medium at the 28th d of culture strongly promoted the cell growth and the formation of the three taxanes in the suspension cells. Compared with those of the control, the cell biomass of the treatments supplemented with phenylalanine and added with sucrose and mannitol at the 28th d of culture increased by 06～08fold, taxol yield by 9～10fold, baccatin III yield by 25～30fold, and 10deacetylbaccatin III yield by 7fold. Addition of sucrose 730 mmol·L-1 at the 28th d of culture significantly promoted the cell growth, but showed little effect on the contents of the three taxanes in the suspension cultures.