类肝素对血管平滑肌细胞增殖的影响

EFFECT OF HEPARINOID ON THE PROLIFERATION OFRABBIT VASCULAR SMOOTH MUSCLE CELLS IN VITRO

以细胞培养法并用肝素等作对照，观察类肝素对兔主动脉血管平滑肌细胞（ＶＳＭＣｓ）增生的影响。结果表明，类肝素在１６～００５ｍｇ·ｍｌ－１浓度下，对ＦＣＳ，ｂＦＧＦ，ＩＬ１刺激细胞增殖均有明显的抑制作用。将ＶＳＭＣｓ在类肝素０２～００５ｍｇ·ｍｌ－１中预孵２４ｈ后弃去药液，再在含ＦＣＳ培养液中继续培养，也能明显抑制细胞增殖。类肝素对ＦＣＳ及ＩＬ１诱导ＶＳＭＣｓ增殖的抑制作用比同浓度的肝素弱，但对ｂＦＧＦ诱导ＶＳＭＣｓ增殖的抑制作用比肝素强。右旋糖酐对ＶＳＭＣｓ增殖无抑制作用，提示抑制作用与分子中含硫酸基有关。

Proliferation of vascular smooth muscle cells(VSMCs) represents an important event in vascular lesion formation. In the present study, we investigated whether heparinoid abstracted from porcine duodenum inhibits the proliferation of rabbit aortic VSMCs in vitro, using the method of colorimetric MTT (tetrazolium). Our results showed that heparinoid at 16～005 mg·ml-1 significantly inhibits VSMCs proliferation induced by fetal calf serum(FCS, 10%), basic fibroblast growth factor(bFGF, 50 ng·ml-1) or interleukin1(IL1, 50 u·ml-1). In inducing with bFGF, the inhibitive effect of heparinoid was more potent than that of heparin, while in inducing with FCS or IL1, the inhibitive effect of heparin was more potent than that of heparinoid. Preincubation with heparinoid at 02～005 mg·ml-1, the vascular smooth muscle cell proliferation induced by FCS was also inbibited. Our results imply that heparinoid may be useful to protect the atherosclerosis and angioplasty restenosis.