金丝桃甙对脑缺血再灌损伤保护作用的实验研究

PROTECTIVE EFFECT OF HYPERIN AGAINST CEREBRAL ISCHEMIAREPERFUSION INJURY

用脑缺血再灌模型和跳台试验，研究了金丝桃甙（Ｈｙｐ）对脑缺血再灌损伤的保护作用。结果表明，在小鼠脑缺血再灌模型上，Ｈｙｐ（５０及１００ｍｇ·ｋｇ－１，ｉｐ）可显著抑制脑组织乳酸脱氢酶（ＬＤＨ）活性的下降；表明，Ｈｙｐ跳台试验对脑缺血再灌致小鼠学习记忆功能障碍有明显的改善作用；在大鼠四血管结扎模型上，Ｈｙｐ可显著抑制脑组织中ＬＤＨ、超氧物岐化酶（ＳＯＤ）及谷胱甘肽过氧化物酶（ＧＳＨＰｘ）活性的降低，减少脑组织脂质过氧化产物丙二醛（ＭＤＡ）和一氧化氮（ＮＯ）的含量的增高，并促进ＥＥＧ变化的恢复。上述结果提示Ｈｙｐ对脑缺血再灌损伤有显著保护作用，其机制可能与其抗自由基、抑制ＮＯ的生成有关。

The protective effect of hyperin (Hyp) against cerebral ischemiareperfusion injury was studied. On the cerebral ischemiareperfusion model in mice, Hyp (50, 100 mg·kg-1, ip) was shown to markedly and dosedependently inhibit the decrease of lactate dehydrogenase (LDH) in cerebrum and improve the learning and memory impairment on the step down test. On the fourvessel occlusion model in rats, Hyp(50 and 100 mg·kg-1, ip) significantly reduced the decreases of glutathione peroxidase(GSHPx), superoxide dismutase(SOD) and LDH activities in the cerebrum. Hyp was also shown to inhibit the increase of nitric oxide (NO) and malondialdehyde (MDA) contents in the cerebrum and promote the recovery of EEG activities. These results suggest that Hyp has protective effect against cerebral ischemiareperfusion injury via attenuating free radical and NO.