丹参的超临界CO_2提取工艺研究

Study on Extraction Process of Salvia Miltiorrhiza Bge.by SFE CO_2

目的筛选丹参的超临界CO2提取最佳工艺条件。方法采用L9(34)正交试验,以丹参酮ⅡA含量为指标进行正交试验,对丹参的超临界CO2提取工艺进行考察,同时将考察结果与传统提取方法进行比较。结果丹参的超临界CO2提取最佳工艺为:取丹参药材粗粉(过20目筛)进行萃取,萃取压力为30 MPa,提取时间为2 h,萃取温度为40℃,解析压力为5 MPa.与传统乙醇回流提取方法相比较,丹参酮ⅡA和丹酚酸B的提取率分别增加至1.25倍和1.58倍。结论运用该工艺提取效率高,稳定性较好,且有效成分提取率高于传统提取方法,适合大生产。

Objective To optimize the extraction process for Salvia miltiorrhiza Bge. by SFE CO2 . Methods The orthogonal experiment was designed and the content of tanshinone Ⅱ A and salvianolic acid B were wed as index to determine the optimum technologic parameters. Results The optimum process was as follows： extracting pressure 30MPa, extracting temperature 40℃, extracting time 2h, separating pressure 5MPa. The extraction rate of tanshinone Ⅱ A and salvianolic acid B were 1.25 and 1.58 times higher than the trenditionerl method using alcohol. Conclusion With the extraction rate higher than the common method by alcohol, the optionnal process is stable, feasible and suitable for production.