人乳头瘤病毒感染、p53 基因突变与子宫颈癌的相关性研究

STUDY OF HPV INFECTION AND p53 GENE MUTATION IN CERVICAL CANCER

应用聚合酶链反应（ＰＣＲ）、单链构像多态性（ＳＳＣＰ）分析银染色法检测了６１例子宫颈癌、５例子宫颈间变、１４例慢性子宫颈炎组织中１６、１８型人乳头瘤病毒（ＨＰＶ１６、１８）感染及ｐ５３基因５、６、７、８外显子的变异。结果显示，子宫颈癌、子宫颈间变及慢性子宫颈炎组织中ＨＰＶ１６、１８阳性率分别为８２％、４０％及１４．３％（Ｐ＜０．０５），子宫颈癌组织中ｐ５３基因突变阳性率为２０％，而子宫颈间变及慢性子宫颈炎组织中未见有突变，其中１２例突变阳性的癌组织中９例呈ＨＰＶ阴性，３例呈ＨＰＶ阳性。提示ＨＰＶ１６、１８感染在子宫颈癌发生中起重要的病因学作用；ｐ５３基因变异可能是部分子宫颈癌变的一个重要原因；高危ＨＰＶ除了通过Ｅ６／ｐ５３蛋白复合物形成使ｐ５３蛋白失活的致癌途径外，可能还存在通过ｐ５３基因突变的致癌途径。

HPV16,18 infection and p53 gene mutations in exons 5￣8 were analysed in 61 cases of cervical cancer,5 cases of cervical atypical hyperplasia and 14 cases of cervicitis.The results showed that the positive rates of HPV16,18 were 82%,40% and 14.3% respectively(P<0.05),and that p53 gene mutation were found in 12 cases of cervical cancer(20%),no mutation was found in other samples.Among mutationpositive cases,9 were HPVnegative,3 were HPVpositive.It suggested that HPV16,18 infection had an important key step in development of cervical cancer,mutation of p53 gene is important in the pathology of some cervical cancer,HPV E6 protein may not only target wild type p53 protein for degradation but also promote p53 gene mutation during carcinogenesis.