广州地区散发性戊型肝炎病毒基因片段序列分析

Nucleic acid sequence analysis of the sporadic hepatitis E virus strains in Guangzhou

目的对广州地区散发性戊型肝炎病毒（ＨＥＶ）作基因片段序列分析。方法用逆转录套式聚合酶链反应（ＲＴ－ｎｅｓｔｅｄＰＣＲ）检测了８例广州地区散发性戊肝患者粪便和血清中的ＨＥＶＲＮＡ，其中３例粪便阳性。对阳性ＰＣＲ产物进行基因克隆、核酸序列分析。结果广州地区Ｇ１、Ｇ２、Ｇ３株与缅甸株（Ｂ）、巴基斯坦株（Ｐ）、墨西哥株（Ｍ）、中国新疆株（Ｃｈ１．１）和广州血清株（Ｇ－９）的核苷酸和氨基酸的同源性均值分别为８０．６７％和８８．６０％（Ｂ）、８１．２５％和８９．２０％（Ｐ）、７７．４５％和８４．８１％（Ｍ）、８１．２５％和８９．２０％（Ｃ）、９７．８５％和９６．２０％（Ｇ）。结论广州ＨＥＶ株有一定程度的变异。

There were sporadic cases of hepatitis E in Guangzhou.In order to analyze the nucleotide sequence of sporadic HEV strain in Guangzhou, RTnested PCR was used to determine HEV RNA in stool and serum samples of 8 patients with hepatitis E.Three stool samples were positive for HEV RNA. The hemology of nucleic acid and amino acid sequences of 3 HEV strains in Guangzhou compared with the Burmese,Pakistani,Mexican,Chinese Ch1.1 and G9 strains were 80.67% and 88.60%(B),81.25% and 89.20%(P),77.45% and 84.81%(M),81.25% and 89.20%(C),97.85% and 96.20%(G),respectively.The results suggest that HEV strains in Guangzhou have genomutation in some extent.