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转录因子Fox01特异性siRNA载体的构建 被引量:1

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摘要 目的构建针对转录因子Fox01 mRNA的特异性siRNA载体。方法依据GenBank中Fox01(NM-002015)的序列,设计并合成针对Fox01的siRNA靶寡核苷酸序列,退火后连接入pSIREN—DNR—DsRed载体,转化扩增后得到重组载体pSIREN—DNR—DsRed—siFox01,并进行序列测定。结果经测序,成功构建pSIREN—DNR—DsRed—siFox01重组载体。结论成功构建针对转录因子Fox01mRNA的特异性SiRNA载体。
出处 《中国实用医刊》 2009年第7期62-63,共2页 Chinese Journal of Practical Medicine
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