乳化剂HLB值对莪术油亚微乳理化性质的影响

Effect of HLB Value on Physicochemical Properties of Curcuma Zedoaria Oil-loaded Submicron Emulsion

目的制备莪术油亚微乳,并考察乳化剂的HLB值对亚微乳理化性质的影响。方法使用高压微射流纳米分散仪制备含不同HLB值乳化剂的莪术油亚微乳;激光粒度及电泳分析仪测定其粒径和表面电位;离心法测定其稳定常数;测定药物在不同HLB值乳化剂的水相中的溶解度,并采用透析法测定亚微乳的体外释放。结果制备得到的莪术油亚微乳粒径在123～154nm之间,粒子带负电。几种乳化剂对药物均有明显的增溶作用,增溶倍数从5.5～8.2倍不等,载药亚微乳的体外释放符合Higuchi方程,且能持续释放48h。结论乳化剂的HLB值不同,制备得到的亚微乳的性质如粒径和稳定性有所不同;随着HLB值增加,乳化剂对药物的增溶作用增强,药物从亚微乳中释放略有加快。

OBJECTIVE To prepare curcuma zedoaria oil-loaded submicron emulsion and investigate the effect of HLB on its physicoehemical properties. METHODS The high pressure homogenization method was employed to prepare cureuma zedoaria oil- loaded submicron emulsion. The particle distribution and zeta-potential were determined by using zetasizer. The stability of the submicron emulsion was investigated by centrifugalization. The solubilities of zedoaria oil in aqueous phase with various HLB value were determined. The dialysis method was established for determination of drug release behavior of cureuma zedoaria oil in the submicron emulsion. RESULTS Curcuma zedoaria oil-loaded submieron emulsion was charged negative and with the mean particle size between 123 nm and 154 nm. Solubilizations were observated obviously with emulsifiers of various HLB value, and solubilization muhiples were between 5.5 and 8.2. In vitro drug release experiments indicated the drug release from submicron emulsion was lasted to 48 hours, and the release behavior was consistent with Higuchi equation. CONCLUSION The cureuma zedoaria oil-loaded submieron emulsions with various HLB value of emulsifier were prepared. Their physicoehemical properties such as particle distribution and stability were different accompany with various HLB value of emulsifiers. Moreover, as HLB value of emulsifiers increased, more solubilizations were observated, and accelerated releases were observated when released in vitro, and was represented a sustained release.