大鼠脑中血浆硒蛋白P cDNA克隆

TX SELENOPROTEIN P cDNA OF RAT BRAIN

方法：用大鼠肝中血浆硒蛋白ＰｃＤＮＡ为探针，对λＺＰＡＩ大鼠脑ｃＤ－ＮＡ文库进行筛选，对阳性克隆进行了核酸序列测定。结果：脑中ｃＤＮＡ中蛋白编码区与肝中ｃＤＮＡ的编码区完全一致，但在５ｕｔｒ和３ｕｔｒ存在差异。结论：脑中血浆硒蛋白ＰｃＤＮＡ的发现说明脑本身可合成血浆硒蛋白Ｐ，这一结果不支持血浆硒蛋白Ｐ的硒转运蛋白功能说。

For establishing that selenoprotein P is synthesized in brain,a λZAPII rat brain library was screened with selenoprotein P cDNA of liver.Positive clones were obtained and sequenced.The selenoprotein P cDNA of brain(2041 bp with poly A tail)is shorter than the cDNA of liver(3800 bp).Comparing the sequence of selenoprotein P cDNA coming from brain with the sequence of cDNA of liver,the open reading frame is the same,even though there are some differences in 5utr and 3utr between the two cDNA.The results indicate the selenoprotein P can be expressed in brain too.This results do not support the proposed function of selenoprotein P as selenium transport protein.