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大鼠脑中血浆硒蛋白P cDNA克隆 被引量:1

TX SELENOPROTEIN P cDNA OF RAT BRAIN
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摘要 方法:用大鼠肝中血浆硒蛋白PcDNA为探针,对λZPAI大鼠脑cD-NA文库进行筛选,对阳性克隆进行了核酸序列测定。结果:脑中cDNA中蛋白编码区与肝中cDNA的编码区完全一致,但在5utr和3utr存在差异。结论:脑中血浆硒蛋白PcDNA的发现说明脑本身可合成血浆硒蛋白P,这一结果不支持血浆硒蛋白P的硒转运蛋白功能说。 For establishing that selenoprotein P is synthesized in brain,a λZAPII rat brain library was screened with selenoprotein P cDNA of liver.Positive clones were obtained and sequenced.The selenoprotein P cDNA of brain(2041 bp with poly A tail)is shorter than the cDNA of liver(3800 bp).Comparing the sequence of selenoprotein P cDNA coming from brain with the sequence of cDNA of liver,the open reading frame is the same,even though there are some differences in 5utr and 3utr between the two cDNA.The results indicate the selenoprotein P can be expressed in brain too.This results do not support the proposed function of selenoprotein P as selenium transport protein.
出处 《营养学报》 CAS CSCD 北大核心 1998年第1期1-7,共7页 Acta Nutrimenta Sinica
基金 国家自然科学基金 非教育系统留学回国人员科技活动D类资助
关键词 硒蛋白 CDNA 血浆 selenoprotein brain cDNA
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参考文献5

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同被引文献36

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