硒、大蒜素对糖尿病小鼠抗氧化功能的影响

TX INFLUENCE OF SELENIUM AND ALLITRIDI ON ANTIOXIDANT IN THE DIABETIC RATS

目的：为探讨糖尿病的发病机理，观察糖尿病小鼠心、脑、脾、肾、胰腺的ＧＳＨ－Ｐｘ、ＳＯＤ、Ｈ２Ｏ２ａｓｅ、ＭＤＡ及ＮＯ含量的变化及硒、大蒜素单用与合用对糖尿病小鼠抗氧化功能的影响。结果：糖尿病小鼠心、脾、肾、胰腺ＧＳＨ－Ｐｘ、ＳＯＤ、Ｈ２Ｏ２ａｓｅ活性均呈下降或呈下降趋势，ＭＤＡ含量升高，其中脾和胰腺的变化较为显著，除心脏ＮＯ含量降低外，脾、肾、胰腺含量明显升高，对脑无显著影响。硒、大蒜素对不同抗氧化酶活性影响各不相同，硒主要提高ＧＳＨ－Ｐｘ及Ｈ２Ｏ２ａｓｅ活性，而大蒜素对ＳＯＤ酶活性影响较大，但它们均能降低脾、肾、胰腺ＭＤＡ和ＮＯ含量。结论：糖尿病的主要病变部位在脾、肾、胰腺，硒、大蒜素能改善其抗氧化功能。

The influence of selenium and allitridi on GSHPx, SOD, hydngen peroxidase activity and MDA content NO content were studied. The results were as follows:1.MDA content in the heart, spleen, kidney, pancreas of the alloxan diabetic rats were increased significantly, but brain did not change obviously and selenium, allitridi could reduce MDA content in the spleen, kidney, pancreas of diabetic rats, but was of no effect on heart and brain.2.GSHPx activity in heart, spleen, kidney, pancreas of the alloxan diabetic rats were decreased sharply, and selenium could increase GSHPx activity in spleen and pancreas but allitridi could only increase it in spleen.3.SOD activity in heart, kidney, pancreas of the alloxan diabetic rats were decreased significantly, selenium had no effect on SOD in the organs, but allitridi could increase SOD activity in heart, brain, kidney, pancreas but no effect on spleen.4.H2O2ase activity in heart, brain, spleen, kidney, pancreas of diabetic rats were decreased sharply, and selenium could increase H2O2ase activity in the organs, but allitridi only could increase H2O2 ase activity in heart.5.NO content were reduced in heart and increased in spleen, kidney, pancrease significantly, but no effect on brain. Selenium and allitridi could decrease NO content in spleen, kidney, pancreas and increase NO content in heart. In a word, selenium and allitridi could improve the antioxidant in the alloxan diabetic rats, and the antioxidant effect of selenium on spleen, pancreas were better than allitridi. The effects of selenium used in combination with allitridi were not better than it alone.