海藻硫酸多糖抗氧化与抗肿瘤作用的实验研究

TX THE EXPERIMENTAL STUDY ON THE ANTIOXIDATIVE AND ANTITUMOR EFFECTS OF SULFATED POLYSACCHARIDES FROM SEAWEEDS

目的：观察海藻硫酸多糖（ＳＰ）对正常及荷瘤小鼠肝匀浆及红细胞中ＳＯＤ，ＧＳＨ－Ｐｘ活性及脂质过氧化产物ＭＤＡ含量的影响，探讨其抗氧化与抗肿瘤之间的关系。结果：移植肿瘤Ｓ１８０使肝组织中ＳＯＤ，ＧＳＨ－Ｐｘ活性分别由１１３．７３±８．１７降至７１．４１±１０．９８ｍｇ／Ｌ（Ｐ＜０．０５），３２．３４±０．０５降至２９．９６±０．１６Ｕ（Ｐ＜０．０１），ＭＤＡ含量由０．２３４±０．００１增至０．２８０±０．０１２（Ｐ＜０．０５），腹腔注射或口服ＳＰ则使ＳＯＤ，ＧＳＨ－Ｐｘ活性增至１１８．７７±１８．９２ｍｇ／Ｌ（Ｐ＜０．０５），３６．５８±１．０６Ｕ（Ｐ＜０．０１），ＭＤＡ含量降至０．１０４±０．０３９（Ｐ＜０．０１）。且腹腔注射ＳＰ１００ｍｇｋｇ－１ｄ－１×１０ｄ可使肿瘤抑制率达３９．１３％，口服ＳＰ２００ｍｇｋｇ－１ｄ－１×１０ｄ也能使肿瘤抑制率达２８．０４％。结论：ＳＰ抑瘤作用的机理可能与抗氧化有关。

The purpose of this study was to investigate the antioxidative and antitumor effects of sulfated polysaccharides from seaweeds(SP) and the relationship between them. The results showed that when given intraperitoneally(ip) or per os(po), SP could significantly inhibit the growth of Sarcoma180, the inhibition rates being 38.9% (SP 50mg·kg-1 ip) and 2804% (SP 200mg·kg-1 po) respectively. Tumorbearing decreased the SOD and GSHPx activities from 113.73±8.17 to 71.41±10.98 mg·L-1 (P<0.05) and from 32.34±0.05 to 29.96±0.16 U (P<0.05), and increased the MDA level from 0.234±0.001 to 0.280±0012 (P<0.05) in liver, while SP increased SOD and GSHPx activities to 118.77±1892mg·L-1 (P<0.05, SP 200mg·kg-1 po) and 36.58±1.06U (P<0.01, SP 100mg·kg-1 ip) respectively and decreased MDA level to 0.104±0.039 (P<0.01, SP 50mg·kg-1 ip). The results suggest that the antitumor effect of SP may be releated to antioxidation.