健康成人口鼻呼出气一氧化氮含量测定及其影响因素分析

Measurement of exhaled nitric oxide in healthy Chinese

目的呼出气中一氧化氮（nitric oxide，NO）含量是反映上下气道炎性反应程度的指标，为此测定健康成人经口和经鼻呼出气中NO含量，得到相应的参考值并分析其影响因素。方法健康成人80名（男20名，女60名），年龄18～44岁，中位数年龄25岁。采用耐尔斯（NIOX）呼出气NO测定系统，分别测定口呼出气和鼻呼出气中NO含量，进而采用多元线性回归分析法，研究性别、年龄、身高、体质量指数（body mass index，BMI）、测量时间和环境NO含量对检测结果的影响。结果①健康成人口呼出气NO含量为（17±8）×10^-9[95％可信区间：（0．3—33）×10^-9]；鼻呼出气NO含量为（819±211）×10^-9[95％可信区间：（400～1238）×10^-9]。②口呼出气NO含量与受试者身高呈正相关，回归方程：Y（口呼出气NO含量）=-58．524±0．457X（身高，cm），t=-2．985，P〈0．01。受试者的年龄、性别、BMI、测量时间和环境NO含量对口呼出气NO含量无显著影响。③健康成人鼻呼出气NO含量与年龄呈正相关，与性别呈负相关，回归方程：Y（鼻呼出气NO含量）=760．245±9．417X1（年龄）-111．222X2（性别），t=5．188，P〈0．01。患者鼻腔左右侧别、身高和BMI、测量时间和环境NO含量对鼻呼出气NO含量无显著影响。结论口、鼻呼出气中NO含量测定操作简便、灵敏度较高，是一种非侵袭性的临床检测手段，正常参考值可受到受试者性别、身高和年龄等因素的影响。

Objective To obtain the normal values of nitric oxide （NO） exhaled throuth nose and mouth in healthy Chinese adults by measuring exhaled NO and analyzing the influencing factors. Methods Eighty healthy Chinese adults were recruited, including 20 males and 60 females. The age ranged from 18 to 44 years old. Chemiluminescence analyzer （NIOX） was used to obtain the values of exhaled NO throuth nose and mouth. The relativity between NO and gender, age, height, body mass index, time, ambient NO were analyzed with Multiple linear regression and correlation. Results Exhaled NO values were （ 17 ± 8） ×10^-9 and correlated significantly with height. Regression equation： Y（ exhaled nitric oxide） = -58.524 ± 0. 457X（ height, cm）, t = - 2. 985, P 〈 0. 01. Transnasal NO values were （ 819 ± 211 ） ×10^-9 and correlated significantly with age and gender. Regression equation： Y （nasal nitric oxide ） = 760. 245 ± 9. 417X1 （age） - 111. 222）（2 （gender） ,t =5. 188,P 〈0. 01. Conclusions Exhaled NO normal values were 17 ×10^-9 and Transnasal NO normal values were 819 ×10^-9. Exhaled NO correlated positively with height. Transnasal NO correlated positively with age and negatively with gender