人肝细胞癌中整合素α5、β1亚基和纤维连接蛋白mRNA表达的生物学意义

EXPRESSION OF INTEGRINα5 、β1 SUBUNIT AND FIBRONECTIN mRNA IN HUMAN HEPATOCELLULAR CARCINOMA

用Ｎｏｒｔｈｅｒｎ印迹杂交对１５份人肝细胞癌（ＨＣＣ）、５例癌旁肝硬变和３例正常肝组织标本中整合素α５、β１亚基和纤维连接蛋白（ＦＮ）ｍＲＮＡ表达作杂交分析。结果：低分化ＨＣＣ癌组织中整合素α５、β１亚基和ＦＮｍＲＮＡ表达水平明显低于高分化ＨＣＣ及癌旁和正常肝组织（Ｐ＜０．０１）；ＨＣＣ伴肝内浸润和（或）转移组癌组织内３种ｍＲＮＡ水平较无浸润转移组低，差异有显著性意义（Ｐ＜０．０５）；５例伴肝内转移的ＨＣＣ癌组织ＦＮｍＲＮＡ呈异质性表达（５．０ｋｂ位置）。提示：ＦＮ及其受体整合素α５、β１基因转录水平的改变与ＨＣＣ细胞分化、浸润和转移密切相关；异质性ＦＮｍＲＮＡ及其编码的ＦＮ蛋白可能在ＨＣＣ转移中有意义。

The expression of integrin α5 、β1 subunit and FN mRNA was detected by Northern blot in 15 cases of HCC tissues, 5 cases of paracancerous cirrhotic liver tissues and 3 cases of normal liver tissues. The levels of expression of integrin α5 , β1 and FN mRNA in poorlydifferentiated HCC were decreased or absent in contrast to the paracancerous liver cirrhotic/normal liver tissues and the welldifferentiated HCC(P<0.01, respectively). In all cases of HCC tested,the expression of integrin α5, β1 and FN mRNA was lower in 8 cases of HCC with focal invasion and/or intrahepatic metastasis than that in the cases without invasion or metastasis(P<0.05). In addition, the heterogeneous expression of FN mRNA(on 5.0 kb position) was found in tumor tissues from 5 cases of HCC with intrahepatic metastasis. The results indicated that the altered expression of integrin α5, β1 and FN proteins at gene transcription level is well associated with the cell differentiation, invasion and metastasis of HCC. The unusual FN mRNA and its encoed FN protein might have some biologic significance in the process of HCC metastasis.