水稻APC/C辅激活子CDH1同源基因OsCCS52B的表达研究(英文)

Expression Studies of Rice APC/C Co-activator CDH1 Homologue OsCCS52B during Cell Cycle

利用同源克隆的方法得到水稻的泛素连接酶APC/C辅助激活子CDH1的同源基因OsCCS52B.通过蛋白质序列分析发现OsCCS52B和苜蓿及拟南芥中的AtCCS52B(细胞周期转换开关基因)基因同源性最高;RNA原位杂交实验研究发现,OsCCS52B基因在减数分裂期间的表达存在一个高-低-高的波动变化.由于OsCCS52B表达变动的这个模式和减数分裂M-M(细胞分裂-细胞分裂)的转化过程中对受CDH1调控的细胞周期激酶CDKA活性的要求相一致,所以推测水稻的OsCCS52B基因参与了水稻减数分裂M-M转换期间对染色体复制的调控.同时,RNA原位杂交实验显示,OsCCS52B在核内复制旺盛的组织如根尖分生组织和穗下节的分生区和伸长区表达强烈,证明OsCCS52B可能参与了水稻的核内复制.

Through Blast research, OsCCS52B gene of the rice was identified as one of the CDH1 homologue. CDH1 is synonymously called the cell cycle-switch （CCS52） in the plant. The deduced amino acid sequences and phylogenetic analysis revealed that OsCCS52B is the homologue of CDH1. RNA in situ hybridization showed that the expression of rice CDH1 homologue,OsCCS52B oscillates during meiosis. The oscillation of OsCCS52B matches the CDKA activity in meiosis,as CDKA is one of the targets of APC/C^CDHI an essential component in DNA replication. Therefore,we have proposed that OsCCS52B is involved in the transition in rice. RNA in situ hybridizaroot tip. The result of this study suggests that OsCCS52B is also involved in the endoreduplication.