NaCl胁迫对菜用大豆种子游离态多胺含量的影响

Effects of NaCl Stress on Free Polyamine Contents in Vegetable Soybean Seeds

采用蛭石栽培,在100 mmol·L^-1NaCl胁迫下,对耐盐性不同的2个菜用大豆[Glycine max（L.）Merr.]栽培品种的种子膨大及其游离态多胺（PAs）含量的变化进行了研究.结果显示：（1）耐盐品种绿领特早的荚重、种子大小及重量的增加在NaCl胁迫15 d时未受显著影响,而盐敏感品种理想高产95-1＇种子的膨大却受到了显著抑制.（2）绿领特早种子的腐胺（Put）含量在盐胁迫6-15 d时均显著低于对照,理想高产95-1＇在胁迫后期（12d、15 d）才显著低于对照,且降幅低于同期的绿领特早;绿领特早种子的亚精胺（Spd）含量在胁迫中、后期（9-15 d）显著高于对照,理想高产95-1＇在胁迫12 d时无显著变化,其余时期均显著低于对照;NaCl胁迫期间,绿领特早种子精胺（Spm）含量的增幅高于同期的理想高产95-1＇（3）与理想高产95-1＇相比,绿领特早在胁迫期间能维持较低的Put/PAs值和较高的（Spd＋Spm）/Put值.研究结果表明,耐盐菜用大豆绿领特早种子在NaCl胁迫期间能保持较高的Spm含量,并在胁迫中、后期保持较高的Spd含量,同时具有较强的Put向Spd和Spm的转化能力,这可能是其耐盐性较强的重要原因之一.

With vermiculite culture, the changes in seed filling and seed polyamine contents of two vegetable soybean [Glycine max （L.） Merr. ] cultivars with different salt tolerance were studied under 100 mmol·L ^-1 NaCI stress. The results showed that the pod fresh and dry mass,seed size, seed fresh and dry mass of ‘Lulingtezao’ resistant to salt stress were not influenced significantly at the 15th day of salinity, while those of ‘Lixianggaochan 95-1’ susceptible to salt stress decreased remarkably in comparison with its control. The putrescine （Put） content in seeds of ‘Luilingtezao’ was significantly lower than that of its control at the 6th, 9th, 12th and 15th days of salinity, while that of ‘Lixiang gaochan 95-1 ’ was decreased significantly at later stages of salinity （12,15 d）, but the decreasing extent was lower than that of ‘Luilingtezao’. The spermidine （Spd） content in seeds of ‘Luilingtezao’ increased significantly at middle and later stages of salinity （9-15 d）. However, that of ‘Lixianggaochan 95-1’ reduced significantly except for the 12th day of salinity when its Spd content had no significant difference in comparison with its control. The increasing extent of sperrnine （Spin） content in seeds of ‘Luilingtezao’ was higher than that of ‘ Lixianggao chan 95-1’.‘Luilingtezao＇ maintained lower Put/PAs ratio and higher （Spdq-Spm）/Put ratio in comparison with ‘Lixianggaochan 95-1＇ during NaC1 stress period. These results indicated that ‘Luilingtezao’ maintained higher Spm contents during NaCl stress period and higher Spd contents at middle and later stages of salinity,and kept higher capability for the conversion of Put to Spd and Spm. This might be one of the important reasons for higher salt tolerance of ‘Luilingtezao’.