脂质体介导血管内皮生长因子基因促进大鼠皮瓣早期断蒂的观察
被引量:1
摘要
1材料与方法
1.1重组质粒的制备pcDNA血管内皮生长因子(VEGF)165(华中科技大学同济医学院杨操博士提供)含VEGF165基因片段,位于BamH I和EcoR I 2个酶切位点之间。将其转入大肠杆菌HB101感受态细胞,挑选抗氨苄西林克隆进行小量快速提取,
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2009年第2期144-145,共2页
Chinese Journal of Burns
基金
宁夏自然科学基金(NZ0671)
参考文献8
-
1Zhang F, Richards L, Angel MF, et al. Accelerating flap maturation by vascular endothelium growth factor in a rat tube flap model. Br J Plast Surg,2002,55(1) :59-63.
-
2Carmeliet P. VEGF gene therapy: stimulating angiogenesis or angioma-genesis? Nat Med ,2000,6(10) :1102-1103.
-
3Taub PJ, Marmur JD, Zhang WX, et al. Lacally administered vascular endothelial growth factor cDNA increases survival of ischemic experimental skin flaps. Plast Reconstr Surg, 1998,102 ( 6 ) : 2033- 2039.
-
4Gurunluoglu R, Ozer K, Skugor B, et al. Effect of transfection time on the survival of epigastric skin flaps pretreated with adenovirus encoding the VEGF gene. Ann Plast Surg,2002,49(2) :161-169.
-
5Giunta RE, Holzbach T, Taskov C, et al. AdVEGF165 gene transfer increases survival in overdimensioned skin flaps. J Gene Med, 2005,7(3) :297-306.
-
6成铤,赵敏,戚可名,王法刚,李桂林,王阳.电脉冲介导的血管内皮细胞生长因子基因治疗对大鼠随意型皮瓣成活的影响[J].中华医学美学美容杂志,2006,12(2):101-104. 被引量:1
-
7王立夫,岑瑛,肖海涛.重组腺病毒介导的血管内皮生长因子165基因增强大鼠横形腹直肌肌皮瓣活力的研究[J].中国修复重建外科杂志,2005,19(8):617-621. 被引量:9
-
8Taub PJ, Marmur JD, Zhang WX, et al. Effect of time on the viability of ischemie skin flaps treated with vascular endothelial growth factor (VEGF) cDNA. J Reconstr Microsurg, 1998, 14 (6) :387-390.
二级参考文献20
-
1汪良能 高学书.整形外科学[M].北京人民卫生出版社,1989.324-332.
-
2Corral CJ, Siddiqui A, Wu L, et al. Vascular endothelial growth factor is more important than basic fibroblastic growth factor during ischemic wound healing. Arch Surg, 1999,134(2):200-205.
-
3Padubidri A, Browne E Jr. Effect of vascular endothelial growth factor (VEGF) on survival of random extension of axial pattern skin flaps in the rat. Ann Plast Surg, 1996,37(6):604-615.
-
4Ozgentas HE, Shenaq S, Spira M. Development of a TRAM flap model in the rat and study of vascular dominance. Plast Reconstr Surg, 1994,94(7):1012-1019.
-
5Tsukamoto H, Wells D, Brown S, et al. Enhanced expression of recombinant dystrophin following intramuscular injection of Epstein-Barr virus (EBV)-based mini-chromosome vectors in mdx mice. Gene Ther,1999,6(7):1331-1336.
-
6Taub PJ, Marmur JD, Zhang WX, et al. Locally administered vascular endothelial growth factor cDNA increases survival of ischemic experimental skin flaps. Plast Reconstr Surg,1998,102(6):2033-2042.
-
7Hallock GG, Rice DC. Physiologic superiority of the anatomic dominant pedicle of the TRAM flap in a rat model. Plast Reconstr Surg, 1995, 96(1): 111-119.
-
8Lubiatowski P, Gurunluoglu R, Goldman CK, et al. Gene therapy by adenovirus mediated vascular endothelial growth factor and angiopoietin-1 promotes perfusion of muscle flaps. Plast Reconstr Surg, 2002, 110(1):149-159.
-
9Pu LL, Ahmed S, Thomson JG, et al. Endothelial cell growth factor enhances musculocutaneous flap survival through the process of neovascularization. Ann Plast Surg, 1999, 42(3):306-312.
-
10Lubiatowski P, Goldman CK, Gurunluoglu R, et al. Enhancement of epigastric skin flap survival by adenovirus mediated VEGF gene therapy. Plast Reconstr Surg, 2002,109(6):1986-1993.
共引文献8
-
1朱明海,梁国辉,张颖,冯峰,张天健.胸腹壁随意皮瓣联合腹直肌肌瓣修复胸壁缺损[J].中国修复重建外科杂志,2006,20(8):832-834. 被引量:4
-
2张波,廖毅.血管内皮生长因子在促进皮瓣血供的应用[J].泸州医学院学报,2006,29(4):371-373.
-
3张琰,毛军胜,王炳臣,张凯,石恩东,林咏杰,黄抗美,蔡锦方.血管内皮生长因子基因治疗联合外科延迟法改善大鼠腹壁超范围轴型皮瓣成活的研究[J].中国修复重建外科杂志,2007,21(6):625-628. 被引量:3
-
4孙智勇,王刚,杨晓楠,杨健祥,滕晓颦,殷国前.天然水蛭素对猪随意型皮瓣静脉淤血影响的实验研究[J].中国修复重建外科杂志,2008,22(11):1296-1300. 被引量:11
-
5李明,贾堂宏,龚维明,杜红霞,刘淑恒,郭舒亚,张庆国,陶世强.重组人促红细胞生成素联合高压氧对移植大鼠背部逆行超长缺血皮瓣存活率的影响[J].中国组织工程研究与临床康复,2009,13(15):2845-2848. 被引量:4
-
6吴莉,沈江涌,杨美玲,杨桂珍.局部应用血管内皮生长因子基因对大鼠皮瓣不同部位作用的实验研究[J].宁夏医学杂志,2010,32(11):982-984. 被引量:1
-
7吴莉.脂质体介导血管内皮生长因子基因促进大鼠皮瓣成活的实验研究[J].医学理论与实践,2012,25(9):1009-1013.
-
8李浙峰,高伟阳,陈时益,李俊杰,闫合德,李志杰,林丁盛.吡格列酮药物延迟对大鼠跨区穿支皮瓣成活的影响[J].中国修复重建外科杂志,2014,28(6):701-706. 被引量:2
同被引文献16
-
1李菲菲,米立国.VEGF_(165)对脱细胞真皮基质血管化影响的研究[J].大连医科大学学报,2009,31(1):18-22. 被引量:6
-
2Breier G, Albrecht U, Sterrer S, et al. Expression of vascular en- dothelial growth factor during embryonic angiogenesis and endo- thelial cell differentiation. Development, 1992,114 (2) :521-532.
-
3Koch AE, Harlow LA, Haines GK, et al. Vascular endothelial growth factor. A cytokine modulating endothelial function in rheu-matoid arthritis. J Immunol, 1994,152 (8) :4149-4156.
-
4Ferrara N, Gerber HP, LeCouter J. The biology of VEGF and its receptors. Nat Med, 2003,9(6): 669-676.
-
5Zhang J, Ding L, Zhao Y, et al. Collagen-targeting vascular en- dothelial growth factor improves cardiac performance after myocar- dial infarction. Circulation, 2009,119 ( 13 ) : 1776-1784.
-
6Yoo YG , Na TY, Yang WK, et al. 6-Mercaptopurine, an acti- vator of Nur77, enhances transcriptional activity of HIF-lalpha resulting in new vessel formation. Oncogene, 2007,26 ( 26 ) : 3823-3834.
-
7Narantuya D, Nagai A, Sheikh AM, et al. Human microglia transplanted in rat focal ischemia brain induce neuroprotection and behavioral improvement. PLoS One, 2010,5 (7) : el 1746.
-
8Gao J, Liu J, Gao Y, et al. A myocardial patch made of collagen membranes loaded with collagen-binding human vascular endothe- lial growth factor accelerates healing of the injured rabbit heart. Tissue Eng Part A, 2011,17(21/22) :2739-2747.
-
9Yan X, Chen B, Lin Y, et al. Acceleration of diabetic wound healing by collagen-binding vascular endothelial growth factor in diabetic rat model. Diabetes Res Clin Pract, 2010,90 (1): 66-72.
-
10McGuane JT, Danielson LA, Debrah JE, et al. Angiogenic growth factors are new and essential players in the sustained relaxin vaso- dilatory pathway in rodents and humans. Hypertension, 2011,57 (6) :1151-1160.
-
1葛华,崔恒,尤宗兵,钱和年,冯捷,付天云.一种经济快速提取组织RNA的方法[J].中华病理学杂志,1999,28(6):460-460.
-
2黄书润,李小毅,刘立飞,苏金荣,何家坤.线扎逐步缩窄蒂部法行皮瓣早期断蒂的实验研究及临床应用[J].中华外科杂志,2006,44(11):762-764. 被引量:8
-
3柳晖,李新强,陈武鹏,胡检,徐华,张显文.VEGF转染VEC促进移植皮瓣血管化的实验研究[J].现代医院,2009,9(1):17-20. 被引量:1
-
4林玉丹,黄书润,邱双双,李丽香,沈秋凤.93例Ⅲ度烧伤患者分段结扎皮瓣早期断蒂的护理[J].中华护理杂志,2011,46(10):956-957. 被引量:1
-
5朱华锋,汪春兰,赵宇,曹东升,丁浩,王帮河,王伟,王兴宇,姚远.应用碱性成纤维细胞生长因子基因治疗促进大鼠早期断蒂皮瓣的成活[J].安徽医科大学学报,2006,41(4):386-388. 被引量:3
-
6鲁晓波,郝建伟,王兆强.皮瓣缺血训练后碱性成纤维细胞生长因子及微血管密度的变化[J].中华显微外科杂志,2006,29(1):48-50. 被引量:4
-
7吴银生,沈江涌,姚明,李津宁,赵巍,苏荣,陆安民.脂质体介导血管内皮生长因子对不同时间断蒂大鼠随意型皮瓣的影响[J].中华整形外科杂志,2009,25(2):120-124. 被引量:4
-
8史春艳,王舒,李福秋.CXCR4在皮肤基底细胞癌中的表达及意义[J].中国老年学杂志,2016,36(20):4980-4981. 被引量:2
-
9姚娟,金庆文,侯熙德,赵中,王宁山,陈荣.结核杆菌DNA三种快速提取方法评价[J].临床检验杂志,1994,12(4):200-200. 被引量:1
-
10郑智,娄宇,赵璐,吴晓伟.舒氨新治疗急性淋巴细胞性白血病继发感染的疗效[J].上海医药,1997(11):13-14.