应用微量细胞培养法检测成人病毒性心肌炎病原的研究

Detection of virus etiology in adult myocarditis by microwell cell cultrue

1988年以来，我们采集114例病毒性心肌炎病人和40例健康人血清和咽拭子标本。用微量细胞培养法检测柯萨奇B（CoxB）病毒中和抗体和病毒鉴定检查。结果心肌炎组中59例双份血清（急性期和恢复期血清），CoxB中和抗体比原来水平增高4倍以上者30例；另外55份单份血清（急性期血清）CoxB中和抗体≥1：320者21例。对照组（40例健康人）双份血清CoxB中和抗体升高4倍以上者7例。二者的P＜0．001，有非常显著性差异。病毒分离结果：心肌炎组分离出14株CoxB病毒，对照组未分离出病毒。血清学阳性和病毒分离阳性共65例，二者有病原学价值占57．0％。

Serum and throat samples of 114 patients with myocarditis and 40 normal adults were tested for the detection of neutral virus antidody and identification of viral isolation for Coxsakie virus B (CoxB) by microwell cell culture. The results showed , 30 out of 59 paired sera (acute phase and convalescent sera) increasedtheir titer, CoxB antibody ≥ 4; 21 out of 55 single serum (acute phase) were CoxB antibody≥1: 320. Thenormal control group: paired serum of CoxB antibody≥4, only 7 samples inceased 1 P<0. 001. There wassignificance of difference between two groups.Viral isolation showed that in myocarditis group 14 strains CoxB viruses were isolated and in normalgroup no strain was isolated. Total positive of serology and viral isolation was 65 samples, and value of etiology diagnosis accounted for 57 %.