摘要
目的观察紫外线照射对阴沟肠杆菌和表皮葡萄球菌生物学特性的影响。方法采用紫外线照射诱导,通过抑菌试验法和生化鉴定方法对诱变后细菌耐药性及生化特性进行了观察。结果阴沟肠杆菌和表皮葡萄球菌的原代菌耐受紫外线照射最长时间均为7 min,阴沟肠杆菌随着紫外照射的代数增多而存活率增加。经紫外线照射后的阴沟肠杆菌生化反应有改变,而表皮葡萄球菌生化反应无变化。经紫外线照射后的阴沟肠杆菌药物敏感变化较大,部分耐药性增加;而表皮葡萄球菌药物敏感变化较小。结论阴沟肠杆菌对紫外线的适应能力较表皮葡萄球菌强,紫外线照射可以诱导阴沟肠杆菌出现亚胺培南不稳定性耐药性增加。
Objective To observe the effect of ultraviolet irradiation on biological Characteristics of Aerobacter cloacae and Staphylococcus epidermidis. Methods Bacteriostatic test and biochemical identification method were used to observe the drug resistance and biochemical characteristics of bacteria after ultraviolet irradiation. Results The longest ultraviolet irradiation tolerance time of the original generations of A. cloacae and S. epidermidis was 7 minutes. The survival rate of A. cloacae increased with increasing the generation multiplication after ultraviolet irradiation. The biochemical changes occurred in A. cloacae , but not in S. epidermidis after ultraviolet irradiation. Changes of drug sensitivity that appeared as resistant to some medicines after ultraviolet irradiation occurred in A. cloacae , while little changes of drug sensitivity occurred in S. epidermidis. Conclusions The adaptability of Aerobacter cloacae to ultraviolet irradiation is stronger than that of S. epidermidis. Ultraviolet irradiation can induce A. cloacae to produce the unstable resistance to imipenem.
出处
《中国消毒学杂志》
CAS
2009年第2期158-161,共4页
Chinese Journal of Disinfection
关键词
外线
阴沟肠杆菌
表皮葡萄球菌
照射诱变
生物特性
ultraviolet rays
Aerobacter cloacae
Staphylococcua epidermidis
radiation mutagenesis
biological characteristics
