恒河猴肺内CGRP及CGRP mRNA免疫组织化学和原位杂交的研究

STUDIES ON IMMUNOHISTOCHEMISTRY AND IN SITU HYBRIDIZATION OF CALCITONIN GENE-RELATED PEPTIDE AND ITS mRNA IN LUNGS OF Macaca mulatta

本实验采用免疫组织化学ＡＢＣ－ＧＤＮ法和地高辛标记寡核苷酸探针原位杂交技术，研究了降钙素基因相关肽（ｃａｌｃｉｔｏｎｉｎｇｅｎｅ－ｒｅｌａｔｅｄｐｅｐｔｉｄｅ，ＣＧＲＰ）及其ｍＲＮＡ在恒河猴肺内的表达。结果显示ＣＧＲＰ分布于恒河猴肺内各级支气管粘膜上皮的神经内分泌细胞（ｎｅｕｒｏｅｎｄｏｃｒｉｎｅｃｅｌｓ，ＮＥＣ）和神经上皮样小体（ｎｅｕｒｏｅｐｉｔｈｅｌｉａｂｏｄｉｅｓ，ＮＥＢ）中。ＣＧＲＰ杂交阳性细胞的分布与免疫组织化学的结果相同。ＣＧＲＰｍＲＮＡ杂交信号均匀分布于整个细胞质，而ＣＧＲＰ免疫反应物仅在神经内分泌细胞的基部更明显，说明肽合成后聚集在基部。免疫组织化学还发现走行于肺内支气管和血管外周的神经纤维亦呈ＣＧＲＰ－ＩＲ阳性。相邻切片法还证实，ＣＧＲＰ与５－羟色胺（５－ＨＴ）共存于ＮＥＣ和ＮＥＢ中，说明二者在功能上可能具有相关性。

お? The occurrence of calcitonin gene-related peptide(CGRP)and its mRNA was studied in lungs of Macaca mulatta using ABC-GDN immunohistochemistry and in situ hybridi-zation The results demonstrated that CGRP was located in neuroendocrine cells(NEC)and neuroepithelial bodies(NEB),including all airways from bronchi,bronchioles and alveolar ducts The distribution of CGRP hybridization positive cells was similar to that previously discribed with immunohistochemistry Labelling for CGRP mRNA was evenly distributed over the cytoplasm,whereas CGRP was usually of high intensity toward the base of neuroendocrine cells,suggesting basal accumulation of synthesized peptide The nerve fibers in walls of the intrapulmonary bronchi and blood vessels were also CGRP positive Adjacent sections immunostained with antibodies to CGRP and 5-hydroxytryptamin(5-HT)proved the coexistance of CGRP and 5-HT in NEC and NEB This finding suggests the possibility of functional interaction of CGRP with 5-HT