糙苏根的化学成分研究

Chemical constituents from roots of Phlomis umbrosa

目的:研究糙苏根的化学成分。方法:用硅胶柱色谱、凝胶柱色谱等多种柱色谱分离,制备高效液相色谱纯化,得到单体化合物,用各种有机波谱鉴定化合物结构。结果:分离得到13个化合物,鉴定为齐墩果酸(oleanolic acid,1),2α-羟基乌苏酸(corosolic acid,2),3β,23-二羟基-12-烯-28-齐墩果酸(hederagenin,3),2α,3β,23-三羟基-12-烯-28-齐墩果酸(arjunolicacid,4),2α,3β,23,24-四羟基-12-烯-28-齐墩果酸(belleric acid,5),3β-hydroxy-29-al-12-en-28-oleanoic acid(6),butyrospermol(7),β-谷甾醇(β-sitosterol,8),β-胡萝卜苷(daucosterol,9),咖啡酸(caffeic acid,10),3-甲氧基-4-羟基-苯甲酸(vanillic acid,11),对羟基苯甲酸(p-hydroxy-benzoic acid,12),3,4-二羟基-苯甲酸(3,4-dihydroxy-benzoic acid,13)。结论:化合物3～9,11～13为首次从糙苏中分离得到的化合物。

Objective： To study the chemical constituents of Phlomis umbrosa. Method： Chemical constituents were isolated by repeated column chromatography （Toyopearl HW-40C and preparative HPLC）. The structures were elucidated on the basis of spectral data analysis. Result： Thirteen compounds were identified as oleanolic acid （1）, corosolic acid （2）, hederagenin （3）, arjunolic acid （4）, belleric acid （5）, 3β-hydroxy-29-a1-12-en-28-oleanoic acid （6）, butyrospermol （7）, β-sitosterol （8）, daucosterol （9）, caffeic acid （10）, vanillic acid （11）, p-hydroxy-benzoic acid （12）, 3, 4-dihydroxy-benzoic acid （13）. Conclusion： Compounds 3- 9, 11-13 were isolated from the plant for the first time.