人参皂甙Rb_1对应激性性行为缺损的保护作用及机制

EFFECT OF GINSENOSIDE Rb1 ON REPEATED STRESSINDUCED SEXUAL DEFICIENCIES IN MALE MICE

用悬吊应激模型，小鼠连续应激小鼠１０ｄ，每天应激一次，并循序增加应激强度，每次应激前３０ｍｉｎ，ｉｐ人参皂甙Ｒｂ１，观察人参皂甙Ｒｂ１对应激性性行为低下的保护作用。结果表明，应激模型组小鼠性行为明显减少，甚至达到缺损的程度，同时血浆睾酮水平明显降低，而Ｒｂ１各剂量组（２５，５，１０ｍｇ·ｋｇ－１）对应激引起的性行为低下及血浆睾酮水平下降均有明显的对抗作用。提示人参皂甙Ｒｂ１对应激性性行为低下有保护作用，其机制可能与Ｒｂ１抑制应激动物血浆皮质酮升高和提高睾酮水平有关。

The effect of ginsenoside Rb1 has been studied on sexual deficiencies induced by repeated hanging stress. Male mice were stressed by hanging once daily(9∶00 am～2∶00 pm) for 10 days(1～3 day hung for 2 h, 4～6 day hung for 3 h, 7～9 day hung for 4 h, 10～11 day hung for 5 h). On day 10, they were exposed to female mice treated with estradiol and progestone and their sexual benaviors (licking, mounting, mating) were assessed at 7∶00～9∶00 pm. The repeated hanging stress was found to reduce sexual behaviors and decrease plasma testosterone level in mice. Treatments with ginsenoside Rb1 (25,5,10 mg·kg-1,ip) 30 min before each stress prevented the repeated stressinduced sexual deficiencies and raised plasma testosterone level. The mechanism of the protective action of ginsenoside Rb1 may be attributed to its action in maintaining normal plasma tesosterone level.