PTHLH在鼻咽癌细胞系及组织中表达上调

Up-Regulation of PTHLH Gene in Nasopharyngeal Carcinoma Cells and Tissues and Its Significance

目的检测PTHLH在鼻咽癌细胞系及组织中的表达水平,从理论上初步探讨其在鼻咽癌发生发展过程中所起的作用。方法实时荧光定量PCR(FQ-PCR)检测PTHLH在7个鼻咽癌细胞系的表达情况,RT-PCR检测PTHLH在鼻咽癌及非癌鼻咽组织中的表达,免疫组化鉴定其在鼻咽癌及鼻咽上皮组织中蛋白水平的表达。结果PTHLH在鼻咽癌细胞系中的表达上调,以HONE1细胞中表达最高。RT-PCR分析显示PTHLH mRNA在鼻咽癌组织中表达明显上调(61.1%),免疫组化检测鼻咽癌石蜡标本(49例)发现,PTHLH蛋白在鼻咽癌组织中表达明显高于鼻咽上皮组织(12例)(P<0.001)。结论PTHLH基因在鼻咽癌细胞及组织中表达增高,提示其可能参与了鼻咽癌的发生发展,有望成为分子生物学诊断的靶点。

Objective To study the expression level of PTHLH in nasopharyngeal carcinoma （NPC）, and predict its significant roles in the genesis and development of NPC. Methods The mRNA expressions of PTHLH gene in six NPC cell lines and immortalized nasopharyngeal epithelial cell line was detected by Real-time Fluorescent Quantitative PCR. RT-PCR and immunohistochemistry methods were used to confirm the expression level of PTHLH in nasopharyngeal carcinoma and non-cancerous nasopharynx （NP） tissues. Results The expression level of PTHLH gene was consistently increased in NPC cell lines, with the highest up-regulation in the HONE1 cell line. RT-PCR results showed the up regulated expression of PTHLH mRNA in 61.1% NPC tissues. The results of 12 NPs and 49 NPCs detected by immunohistochemistry indicated that the protein expression of PTHLH markedly increased in NPC compared to NP. Conclusion The increased expression of PTHLH in NPC cell lines and tissues may be associated with the genesis and progression of NPC. Its might promote the pathogenesis of NPC and also be used as a potential molecular targets for the diagnosis in NPC.