人参皂苷Rg_1对吗啡依赖海马神经元胞内游离钙离子浓度的影响

Effects of Ginsenosides Rg1 on the Intracellular Free Calcium Concentration in Primary Cultured Rat Hippocampal Neurons Subjected to Morphine-Dependence

目的探讨人参皂苷Rg1对吗啡依赖海马神经元胞内游离钙离子浓度(intracellular free Ca2+concentration,[Ca2+]i)的影响。方法采用荧光钙离子指示剂Fluo-3/AM,利用激光扫描共聚焦显微镜观察低剂量(1μmol/L)、中剂量(10μmol/L)、高剂量(100μmol/L)人参皂苷Rg1处理吗啡依赖原代培养大鼠海马神经元后[Ca2+]i的改变。结果低、中、高剂量人参皂苷Rg1单独处理海马神经元前后并没有引起胞内[Ca2+]i的显著改变(P>0.05),而吗啡依赖海马神经元胞内[Ca2+]i较正常海马神经元细胞明显增高(P<0.01)。此外,低、中、高剂量人参皂苷Rg1处理吗啡依赖海马神经元细胞后,均能显著抑制胞内[Ca2+]i的升高(P<0.01),并呈剂量依赖性,Rg1剂量越大,抑制作用越明显(P<0.01)。结论人参皂苷Rg1可以抑制吗啡依赖海马神经元细胞内[Ca2+]i的增高,对吗啡的成瘾性具有明显的拮抗作用,为合理安全有效地应用人参戒毒提供了实验依据。

Objective To investigate the effects of ginsenosides Rg1 on [Ca^2＋]i in primary cultured rat hippocampal neurons subjected to morphine-dependence. Methods After the hippocampal neurons subjected to morphine-dependence were respectively treated with ginsenosides Rg1 （1μmol/L, 10 μmol/L and 100 μmol/L）. Changes of [Ca^2＋]i induced by ginsenosides Rg1 were detected by laser scanning coufocal microscope, by use of Fluo- 3/AM as a calcium fluorescent probe. Results [Ca^2＋]i in normal hippocampal neurons didn＇t have any significant changes after treated with low-, medium-and high-dose ginsenosides Rg1 （P〉0.05）, but morphine induced a significant increase of Ca^2＋]i in hippocampal neurons （P〈0.01）. In addition, low-, medium-and high-dose ginsenosides Rgl could significantly block the increase of Ca^2＋]i induced by morphine in a dose-dependent manner （P〈0.01） o Conclusion The results indicated that ginsenosides Rgl may effectively block the increase of Ca^2＋]i induced by morphine and morphine addiction, and supported that ginseng can be a safe detoxification drug.