聚氨酯与丝素粉体共混膜的血液吸附性

Blood adsorption of polyurethane and silk fibroin powder composite film

背景:为了改善医用聚氨酯的亲水性和血液相容性,利用共混的方法制备聚氨酯/丝素粉体共混膜,但是粉体的加入会显著提高共混膜的吸水性,因此会影响体外血液相容的表征。目的:观察丝素粉体的加入对聚氨酯/丝素粉体共混膜亲水性和血液吸附性能的影响。设计、时间及地点:体外对比观察实验,于2008-08/10在武汉科技学院新型纺织材料绿色加工及其功能化教育部重点实验室和医院实验室完成。材料:蚕丝纤维由浙江桐乡市思源纺织有限公司提供,医用聚氨酯由DOWChemical公司提供。方法:将蚕丝纤维在质量浓度为10g/L,浴比为1∶20的Na2CO3溶液中煮沸3h,洗涤晾干后利用自制磨盘磨制成乳白色粉体而得到非水溶性丝素粉体。将丝素粉体加入到聚氨酯溶液中利用液体交换的方法制备了不同丝素粉体含量(0%,10%,30%,50%,70%)的聚氨酯与丝素粉体共混膜。主要观察指标:扫描电子显微镜观察共混膜表面形貌。计算单位体积共混膜上吸附血液红细胞、白细胞、血红蛋白、血小板的数量。结果:当丝素粉体含量在50%以下时,丝素粉体被包含在聚氨酯大分子中间。当丝素粉体含量在70%时,丝素粉体均匀分布在共混膜表面。随着丝素粉体含量的增加,共混膜吸附血液各组分数量增加。随着时间的延长,共混膜吸附血液的量增加,直到达到动态平衡。在吸附动态平衡过程中,血小板的吸附量下降,主要归功于丝素粉体对其他细胞吸附能力要强于血小板。结论:丝素粉体加入可以明显改善材料的亲水性,并且随着丝素粉体含量的增加,共混膜对血液的吸附能力增强。

BACKGROUND： Polyurethane and silk fibroin powder composite film is discovered in order to improve hydrophilicity and blood compatibility. However, the addition of silk fibroin powder may notably enhance the hydrophilicity of composite film so as to influence superficial syndromes of in vitro blood compatibility. OBJECTIVE： To evaluate the effect of silk fibroin powder at different concentrations on hydrophilicity and blood adsorption of polyurethane and silk fibroin powder composite films. DESIGN, TIME AND SETTING： An in vitro contrast study was performed at the Key Laboratory of Green Processing and Functional Textile of New Textile Materials Ministry of Education, Wuhan University of Science and Engineering between August and October 2008. MATERIALS： Silk fibroin was provided by Siyuan Spinning and Weaving Co., Ltd., Zhejiang, and medical polyurethane was provided by DOW Chemical Company. METHODS： Silk fibroin was boiled in Na2CO3 solution （10 g/L, 1：20） for 3 hours and grinded into ivory powder after washing and drying. Thereafter, silk fibroin powder at different contents （0%, 10%, 30%, 50%, and 70%） was mixed with polyurethane to obtain the composite film using fluid exchange method. MAIN OUTCOME MEASURES： Scanning electron microscopy （SEM） analysis was carried out to obtain the surface morphology of the composite film. And then, numbers of erythrocyte, leukocyte, hemoglobin, and blood platelet were calculated in the unit volume of the composite film. RESULTS： Be lower than 50%, silk fibroin powder was included in giant molecule of polyurethane. Be at 70%, silk fibroin powder was evenly distributed at the surface of the composite film. With the increase of silk fibroin powder content, dosis refracta was increased, and the blood adsorption was also increased till at the dynamic equilibrium. The adsorption for leukocyte, erythrocyte and hemoglobin was increased with the prolonging time but the number of platelet was decreased, because adsorbability to other cells was stronger than platelet. CONCLUSION： The addition of silk fibroin powder may notably improve the hydrophilicity, while the blood adsorption of the composite film is enhanced with the increasing content.