磷灰石-硅灰石生物活性玻璃陶瓷对谷氨酸的吸附

Adsorption properties of apatite-wollastonite bioactive glass-ceramic to glutamic acid

背景:磷灰石-硅灰石生物玻璃陶瓷是近年发展起来的优良骨修复支架材料,可为种子细胞提供良好的生长环境。但是细胞与载体的相互作用及变化机制尚未完全清楚。目的:考察溶胶-凝胶法制备的磷灰石-硅灰石生物活性玻璃陶瓷对谷氨酸的吸附行为,探讨温度、溶液pH值、质量浓度对谷氨酸吸附量的影响。设计、时间及地点:观察实验,于2008-03/09在四川大学材料科学与工程学院完成。材料:用溶胶-凝胶法制备的磷灰石-硅灰石生物活性玻璃陶瓷。方法:将片状磷灰石-硅灰石生物活性玻璃陶瓷浸泡于谷氨酸溶液中,改变谷氨酸溶液质量浓度、pH值、温度等吸附条件。主要观察指标:测量不同条件下的谷氨酸吸附量。结果:①磷灰石-硅灰石生物活性玻璃陶瓷初次对谷氨酸吸附时,属于Freundlich型吸附,包括物理吸附和化学吸附。②谷氨酸较适宜的吸附条件为pH3.4或者8,温度为37℃左右。结论:磷灰石-硅灰石生物活性玻璃陶瓷对谷氨酸的吸附受溶液pH值、温度、谷氨酸质量浓度等因素的影响,且存在一定的规律。

BACKGROUND： Apatite-wollastonite glass ceramic （A-W GC） is recently discovered bone-repaired stent material, and it can provide a good growth environment for seed cells; however, interaction and mechanism of cells and vector remains still unknown. OBJECTIVE： To study the protein adsorption mechanism of A-W GC on glutamic acid, and investigate the effects of temperature pH value, and concentration. DESIGN, TIME AND SETTING： An observational study was performed in the Materials and Engineering Cotlege, Sichuan University between March and September 2008. MATERIALS： A-W GC made by sol-gel method. METHODS： A-W GC sheets were dipped in glutamic acid （Glu） solution, in different temperature, Glu solution concentration and pH. MAIN OUTCOME MEASURES： Characteristics of A-W GC, and glutamic acid adsorption. RESULTS： （1）The adsorption of A-W GC to Glu at the first time could fit for Freundlich equation, indicating that Glu was absorbed physically and chemically. （2） More Glu could be absorbed at pH 3.4 or 8, at 37℃. CONCLUSION： There are effects of temperature, glutamic acid concentration and pH on the adsorption of A-W GC to Glu, showing a regularity.