乳酸菌定量分析方法研究进展

The Research Progress of Quantitative Analysis of Lactic Acid Bacteria

传统的微生物定量检测方法主要运用纯培养技术根据细胞的形态特征或通过使用细胞计数仪来进行定量。近年来,随着分子生物学及生物技术的发展,依赖于特异核酸序列的分子生物学定量技术如:点杂交和狭线杂交、荧光原位杂交、PCR-DGGE以及实时荧光定量PCR技术已经被广泛应用。文中综述了用于乳酸菌定量检测的几种方法,简单概括了各种方法的原理、特点以及其研究应用现状。

The traditional quantitative microbial detection method mainly used cell-culture technology in accordance with the morphological characteristics or the cell count instrument to quantify. In recent years, with the development of molecular biology and biotechnology, the molecular biology quantitative techniques which dependent on the specific sequence of nucleic acid, such as dot blotting and slot blotting, fluorescence in situ hybridization, PCR-DGGE and real--time quantitative PCR technology have been widely used. This paper reviewed some quantitative detection methods of lactic acid bacteria, in which the principle and characteristics of each method are summarized. Meanwhile, perspective and the application progress of LAB quantization were discussed.