微波消解-铬天青S光度法测定海蜇产品中的明矾含量

Determination of Aluminum Residue in Jellyfish Product with Microwave Digestion-CAS Spectrophotometer

在海蜇产品加工过程中需经盐和明矾腌渍处理,以达到脱水和改善产品品质的目的,这种常规加工方式会引起铝元素的残留,因此,明矾含量的监测是海蜇产品质量控制关键环节。该研究改善传统水煮滴定的测定方法,微波消解样品后与铬天青S,溴化十六烷基三甲基铵和聚乙二醇辛基苯基醚反应形成稳定的四元显色体系,明矾含量与其吸光强度成正比。研究结果表明:在595 nm处铝离子浓度在0.1～17.5μg/mL内符合比耳定律,检测方法加标回收率98.69%～102.08%,对应明矾的检测限为0.02%,检测结果与电感耦合等离子体发射光谱法的结果一致。采用微波消解海蜇样品明显提升样品的消化效果,四元显色体系提高了检测的灵敏度和精确度,为海蜇产品中明矾的测定提供了更好的方法,值得推广应用。

Aluminum residue was involved when processing jellyfish pickled with the salt and alum to dehydrate and improve the product quality. Therefore, it＇s of great necessary to fortify monitoring alum residue in jellyfish products for y safety reason. After treated by microwave digester, aluminum in the sample solution react with Chrome azurol S to form a stable quaternary complex in a hexamethylenetetramine buffer medium of ph 6. 7 in the presence of Cetylpyridinium bromide and Octylphenol Polyoxyethylene Ether. At 595nm wavelength ,Beer＇S law was obeyed in the concentration range of 0.1～17.5μg/mL of aluminum. The precision and accuracy of the method was tested. A recovery of 98.69%～102.08% was obtained for spiked samples. The determination limit was 0.02% alum, the equivalent results were observed in the comparisons with ICP-OES method. The accuracy and precision were improved by microwave digestion and chromogenic reaction of quaternary complex. It is a better alum determination method for jellyfish products.