新疆野生樱桃李S-RNase基因分离与鉴定的初步研究

Primary Study on Identification of S-RNase Genes in Wild Myrobalan Plum(Prunus cerasifera Ehrh.)

从4对已报道的李属树种S-RNase基因引物组合中筛选出扩增效果较好的PruC2和PruC4R组合,对新疆野生樱桃李(Prunus cerasifera Ehrh.)的45个株系的基因组DNA进行S-RNase基因特异性PCR扩增,并对其PCR产物进行克隆测序。这些核酸序列及其相应的氨基酸序列在GenBank中进行比对,皆与李属的S-RNase基因有最大同源性,为新疆野生樱桃李的4种S-RNase基因,分别命名为S1(511bp)、S2(787bp)、S3(1859bp)和S4(464bp),在GenBank的登录号分别为EF638726、EF641276、EF661873和EF661874。45个株系中,43个株系的S基因型分别为S1S2、S1S3、S2S3、S2S4和S3S4,而10号和15号株系分别只鉴定了1种S-RNase基因,其S基因型组成有待于进一步研究。

On fourty-five strains of wild myrobalan plum（Prunus cerasifera Ehrh.）,the PCR amplification was carried out with the primer pair PruC2/PruC4R designed according to the conserved sequence of amino acid of Prunus S-RNase.The PCR bands were cloned and sequenced.The sequences were blasted in GenBank which showed them having the maximum identity with Prunus S-RNases,and four S-RNase alleles were identified and named as S1（511 bp）,S2（787 bp）,S3（1 859 bp）and S4（464 bp）alleles respectively.Their accession numbers in GenBank are EF638726,EF641276,EF661873 and EF661874.The S-genotypes of 43 strains were S1S2,S1S3,S2S3,S2S4 and S3S4.However,only one S-RNase allele was identified as S3-RNase on strain 10 and S4-RNase on strain 15,the S-genotypes of strain 10 and 15 remain to be further studied.