云南省建水县番茄上Tospovirus属病毒的初步鉴定

Preliminary Identification of Tospovirus on Tomato in Jianshui County of Yunnan Province

2005—2006年对云南省主要蔬菜种植区昆明、红河和楚雄等3个地区进行调查,获得9份疑似番茄斑萎病毒属(Tospovirus)病毒的样本。通过该属病毒引物(Tospovirus Group Primer)进行RT-PCR扩增,其中1份来自红河州建水县番茄上的样品获得约500bp核酸片段,通过NCBIBLAST网络数据库比对,确定为Tospovirus属的病毒;应用DNAMAN软件与已经公布的Tospovirus属病毒LRNA序列比对分析,从得到的相似性树状图可以看出,该样品与血清组Ⅳ的Tospovirus属病毒聚为一簇。

Nine samples possibly from genus Tospovirus were collected from major vegetable-growing field in Yunnan province,including Honghe,Chuxiong and Kunming,during 2005—2006 period.Around 500 bp fragments were obtained after RT-PCR were executed on 9 samples using Tospovirus Group Primer.The sequence results of around 500 bp fragments were compared in the NCBI BLAST website,one sample from tomato in Honghe County of Yuannan Province was identified as Tospovirus genus.A phylogenetic tree was constructed using DNAMAN software according to the sequence of the sample and their corresponding public L RNA fragment sequences of Tospovirus.The results indicated that this sample obtained in this study fell into the Serogroup Ⅳ.