HOXA10基因启动子区5′CpG岛甲基化在子宫内膜异位症中的作用

Effect of methylation in 5'CpG island of the promoter region of HOXA10 gene on patients with endometriosis

目的检测HOXA10基因启动子区5′CpG岛甲基化状况,探讨其在子宫内膜异位症(EMS)发生中的作用。方法收集2007年9月-2008年5月南方医科大学珠江医院妇产科30例EMS患者作为EMS组,同期25例正常妇女作为对照组,采用甲基化特异性聚合酶链反应(MSP)检测EMS组异位内膜组织(其中Ⅰ-Ⅱ期10例,Ⅲ-Ⅳ期20例)和对照组子宫内膜组织中HOXA10基因启动子区5′CpG岛甲基化情况,并比较其在不同EMS临床分型间的差异。结果30例EMS患者异位内膜组织中检测到程度不等的甲基化,其中20例Ⅲ-Ⅳ期EMS患者中有11例发生甲基化,甲基化发生率为55%,10例Ⅰ-Ⅱ期EMS患者中有1例发生甲基化,甲基化发生率为10%,Ⅲ-Ⅳ期EMS患者异位内膜组织HOXA10基因启动子区5′CpG岛的甲基化发生率高于Ⅰ-Ⅱ期(P<0.05)。25例正常子宫内膜组织均未检测到甲基化。结论HOXA10基因启动子区5′CpG岛甲基化在EMS的发生、发展中可能起着重要作用。

Objective To investigate the methylation status of 5′CpG island of the promoter region of HOXA10 gene, and to explore the pathogenic role of the methylation status on endometriosis. Methods Thirty patients with endometriosis, who were admitted to Zhu jiang Hospital of Southern Medical University from Sep. 2007 to May 2008, and 25 healthy women were involved in present study. Of the 30 patients with endometriosis, 10 were in stages Ⅰ-Ⅱ, and 20 in stages Ⅲ-Ⅳ. The specimens of endometrial tissue were collected from 30 patients with endometriosis and 25 healthy women, and the methylation status in 5′CpG island of HOXA10 gene promoter region was detected by using methylation-specific PCR technique （MSP）. Results Different methylation levels in 5rCpG island in promoter region of HOXA10 gene were detected from the patients in different clinical stage of endometriosis. Methylation in 5′CpG island of the promoter region of HOXA10 gene was observed in 11 of 20 （55%） patients in endometriosis stages Ⅲ-Ⅳ, and 1 of 10 （10%） patients in endometriosis stages Ⅰ-Ⅱ. The methylation level in 5′CpG island of the promoter region of HOXA10 gene of the patients in endometriosis stages Ⅲ-Ⅳ was higher than that of the patients in endometriosis stages 2-11 （P〈0. 05）. Howewer, no methylation was found in the endometrial tissue of 25 healthy women. Conclusion The methylation in 5′CpG island of the promoter region of HOXA10 gene may play an important role in the pathogenesis and development of endometriosis.