嗜麦芽窄食单胞菌中氨基苷类修饰酶基因的研究

Study on Aminoglycoside Modified Enzymes in Stenotrophomonas Maltophilia

目的了解嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia,SMA)氨基苷类修饰酶(aminoglycoside modifying enzymes,AMEs)基因存在情况。方法自临床分离17株嗜麦芽窄食单胞菌,采用聚合酶链反应及序列分析的方法分析6种AMEs基因。结果17株所测嗜麦芽窄食单胞菌中有4株菌aac(6’)-Ⅱ基因阳性,阳性率为23.5%。aac(3)-Ⅱ、aac(3")-Ⅱ、aac(3")-Ⅰ、aac(6’)-Ⅰ、aac(3)-Ⅰ基因均阴性。结论嗜麦芽窄食单胞菌中存在aac(6’)-Ⅱ型氨基苷类修饰酶基因,这种基因常见于假单胞菌属中,证明细菌耐药性的传播。

Objective To investigate the genes of aminoglycoside-modified enzymes （ AMEs ） in Stenotrophomonas maltophilia. Methods Seventeen strains of Stenotrophomonas maltophilia were chnicaUy isolated. The AMEs （6 kinds ） genes of these strains were analyzed using PCR and verified using DNA sequencing. Results The positive rate of genes of aac （ 6″）-llwas 23.5%. All ofaac （3）-Ⅱ,aac （3″）-Ⅱ ,aac （ 3″）-Ⅰ ,aac （ 6″）-Ⅰ ,aac（3）- Ⅰ were negative. Conclusion The gene of aac （ 6′） -Ⅱ was found in Stenotrophomonas maltophilia. The gene commonly detected in Pseudomonas increased resistance against aminoglycoside in Stenotrophomonas maltophilia.