胚胎小鼠大脑c-jun N-末端激酶表达的定量分析

Quantitative Analysis of c-Jun N-terminal Kinases' expression in Mouse Brain During Fetal Development

目的:探讨c-jun N-末端激酶(JNK)在胚胎小鼠脑发育中的作用。方法:按受孕时间将昆明小鼠随机分为6组,分别为胚龄10(E10)、12(E12)、14(E14)、16(E16)、18d(E18)组、新生组(P0),采用免疫印迹技术和光密度分析法对各组小鼠脑JNK进行定量检测,以测得的JNK最大量为1(100%),计算蛋白相对含量。结果:免疫印迹法测得E10、E12、E14、E16、E18及P0组JNK1含量分别为24.57%、46.62%、62.99%、82.06%、100%、89.65%,JNK2/3含量分别为58.89%、51.37%、28.67%、20.33%、6.54%、5.03%。结论:JNK在胚胎发育期间呈明显的变化规律,提示JNK可能在小鼠大脑的发生与发育中起重要作用。

Objective： To study the effects of c-Jun N-terminal Kinases（JNK） in fetal mice brain. Methods： KunMing mice were divided into six groups ：embryonic day of 10（E10）, 12（E12）, 14（E14）, 16（E16）, 18（E18） and 2h after birth （P0）. Using Western blot and photodensitometry detect the expression of JNK. Results： The amounts of JNK1 in six groups were 24. 57%,46.62%,62. 99%,82.06%,100%,89. 65%, respectively; while amounts of JNK2/3 were 58. 89%,51.37%,28. 67%,20. 33%,6.54%,5.03%, respectively. Conclusion： There is a relation between the expression of JNK and brain development in the fetal mice.