β-壳寡糖对营养性肥胖大鼠的减肥作用研究

Weight-reducing Effect of Beta-Chitoligosaccharide on Diet-induced Obese Rats

目的:通过建立营养性肥胖大鼠模型研究β-壳寡糖对营养性肥胖大鼠的减肥作用。方法:通过高脂饮食诱导建立肥胖模型,灌胃给予治疗一个月,观察治疗前后体重、肥胖指数的变化,检测血清生化指标及脂肪,肝脏病理情况。结果:13周末成功建立营养性肥胖模型,灌胃一月后,体重上β-壳寡糖各剂量组与模型组存在显著性差异(P<0.05),其中高剂量组差异非常显著(P<0.01);各用药组均有降低血清总胆固醇(CHO)的作用(P<0.05),其中低、中剂量组及阳性药组差异非常显著(P<0.01),β-壳寡糖低、中剂量组低密度脂蛋白胆固醇(LDLC)与模型组相比差异存在统计学意义(P<0.05),血甘油三酯(TG)及高密度脂蛋白胆固醇(HDLC)各组间则无显著差异(P>0.05),生殖器周及肾周脂肪量,Lee's指数各组明显低于模型组(P<0.05)。结论:β-壳寡糖各组均有减肥作用,从降低体重上看,高剂量组要优于低、中剂量组;从CHO及LDLC上看,低、中剂量组要优于高剂量组。

Objective： To study the effect of high-fat diet on inducing obesity phone rats,and the effect of reducing fat of β-oligosaccharide on these rats. Methods： The experimental rats were given high fat diet for 13 weeks to set up the model, drugs were administered intragastrically to obese rats for 30 days. then bio- chemical indicator such as CHO,TG, HDLC,LDLC were detected, adipose tissue and liver were rapidly collected and HE tinction were used to observe morphological changing of fat cell. Results： After 13 weeks later, there were 30 rats getting 20% weight than normal rats, between which there were significant difference （P〈0. 05）. For one month＇s treatment, lose weight effect were achieved in β-COS groups while fat tissue around genitals,fat around the kidney,volume of white fat cell and lee＇s index in the treated groups were all obviously decreased than those in the model groups. Metabolic profile such as blood lipids has different vari- ance. Obvious difference was observed for CHO with all groups, LDLC with some groups, while no difference was observed for TG and HDLC among groups. Conclusions： All three doses could losing weight, while in losing weight it seems the high dose is better than the middle and low dose.