蓝莓芽诱导与再生研究

Induction and regeneration of blueberry buds

采用改良WPM为基本培养基，研究蓝莓的组培育苗技术。结果表明：以当年生绿枝茎段为外植体可有效获得无菌苗；蓝莓茎段的离体增殖率受到培养基中细胞分裂素的调控，ZT 1-2mg／Li和2ip 10～20mg／L的效果较好，初代培养增殖率3-6倍。继代培养时茎段和叶片也可分别再生无菌苗6-8周继代1次，增殖率可达10倍以上。蓝莓试管苗瓶内生根率不高，0．02～0．2mg／L IBA可诱导蓝莓生根，正常生根率30％～50％。

The modified WPM was taken as the basic medium to study blueberry tissue culture technology. The results showed that the virus-free plantlets could be obtained after taking the green shoots as the explants. The shoots were well proliferated on WPM medium supplemented with ZT 1.0-2.0 mg/L or 2ip 10-20 mg/L, and the multiplication rate of initial culture was 3-6 times. The shoots and leaves asepsis plantlets could also produce virus-free plantlets on the same medium and the multiplication rate of every subculture was over 10 times during 6-8 weeks. The rooting rate in the bottle was not high, which usually was 30%-50%, and 1/2 WPM with the IBA 0.02-0.2 mg/L could be used as rooting medium.