应用于肿瘤细胞基因表达水平分析的微流控芯片

Elaboration of Microfluidic Biochip for Cancer Cell Gene Expression Analysis

近年来快速发展的微流控技术为单细胞基因诊断的基础研究提供了一个有力的平台,微流控芯片具有样品和试剂低消耗、快速实时、大量样本平行处理等优点,还可以使分析过程自动化、防止污染以及完成自动高效的重复实验。本文主要介绍一种用于高通量单细胞基因表达水平分析的微流控芯片技术平台。芯片集成了单细胞捕获、裂解、纯化、逆转录等生物样品处理单元,通过内置气动微阀实现细胞反应腔的独立寻址,从而实现单细胞处理与分析整个过程的自动化操控。实验结果显示了此平台可以实现基因表达水平稳定性的评估,具有应用于肿瘤早期诊断领域的潜力。

Microfluidic devices provide powerful platforms for single-cell transcriptome analysis for many advantages such as highly parallel processing and low consumption of reagents. We have elaborated an integrated system with individual controller for effective single-cell transcriptome analysis. Inside this integrated microfluidic device, the cells are individually manipulated and isolated in an array using micro sieve structures, then transferred into different nanoliter reaction chambers for parallel processing of single-cell transcriptome analysis. The result demonstrates that this platform has the potential for assessment of genetic stability in cancer diagnosis.