斜纹夜蛾核型多角体病毒Ⅱ型DNA甲基化研究

The Genomic DNA Methylation of Spodoptera Litura NucleopolyhedrovirusⅡ

目的:检测斜纹夜蛾核型多角体病毒Ⅱ型是否存在DNA甲基化。方法:选取SpltNPVⅡ的DNA聚合酶序列以及全序中其它三段序列,利用亚硫酸盐修饰直接测序法进行甲基化检测实验。结果:DNA聚合酶中共有246处CG,14处发生甲基化,全序中选取的三段序列只有一段发生3处甲基化。结论:实验表明,SpltNPVⅡ中存在DNA甲基化,其DNA聚合酶甲基化程度高于其它三段序列,且不同序列的甲基化程度不一样,这为以后该病毒的表观遗传学进一步研究奠定了基础。

Objective:To detect the methylation of DNA for Spodoptera litura nucleopolyhedrovirus Ⅱ(SpltNPVⅡ) was existed through the search.Method:Polymerase sequence of DNA and other three sequences in the total were chose,and detect the methylation of DNA by the method of bisulfite-modification.Result:DNA-methylation was taken place in the 14 bureaus of CG among the 246 bureaus of DNA polymerase.And 3 bureaus were taken place in the three sequences which were chose.Conclusion:DNA-methylation was existed in the SpltNPVⅡ.The methylation level of DNA-polymerase of DNA-polymerase was higher than other three sequence,however,difference had different levels.This result was important to future research about the Spodoptera litura nucleopolyhedrovirus.