谷氨酰胺联合泛醇对烧伤家犬肠道损伤的影响及机制研究

Effects of glutamine combined with panthenol on intestinal injury in burned canis familiaris

目的观察应用谷氨酰胺、泛醇及其复方制剂对减轻烧伤家犬肠道损伤的疗效并探讨其机制。方法采用30%体表面积Ⅲ度烧伤家犬模型,动物随机分为正常对照、烧伤对照、泛醇、谷氨酰胺、泛醇+谷氨酰胺5个组,每组6只家犬,观察伤后7d各组动物肠道损伤和修复指标。结果烧伤家犬乙酰胆碱、肠黏膜蛋白含量明显降低,血浆二胺氯化酶(DAO)含量和损伤指数则明显增高(P<0.05),给予谷氨酰胺、泛醇以及复方药物均可明减轻烧伤后肠道损伤,降低血浆DAO活性和黏膜损伤指数(P<0.05);给予泛醇则能明显增加乙酰胆碱合成。与单方组相比,复方制剂组上述各项指标均明显优于单方组。结论烧伤后肠道损伤明显,给予谷氨酰胺能明显减轻肠道损伤,给予泛醇能明显促进乙酰胆碱合成,促进胃肠运动,复方制剂疗效明显优于单方。

Objective To observe the effects of glutamine,panthenol and its compound preparation on lessening intestinal injury in burned canis familiaris.Methods Thirty canis familiaris were randomly divided into five groups,i.e.normal control(C)group,burn control(B)group,glutamine group,panthenol group and its compound preparation group.Burned canis familiaris were inflicted with 30% TBSA full thickness burn injury.Observe the intestinal injury and repair post burn 7 d.Results After burn injury,the activity of plasma diamine oxidase(DAO),intestine mucosal damage index were significantly higher,and the contents of intestine mucosal protein and plasma acetylcholine were remarkable decrease than those of C group(P<0.05).Compare with B group,the activity of plasma DAO,intestine mucosal damage index were markedly decreased in glutamine group,and the plasma acetylcholine contents was significantly increased in panthenol group.Compare with simple recipe group,there were more conspicuous therapeutic effect on compound preparation group.Conclusions The results demonstrate that intestinal histological structure is significantly damage after burn injury,glutamine supplement is beneficial to decreasing intestinal injury,and panthenol administration could remarkable promote acetylcholine synthesis.There are more conspicuous therapeutic effect with glutamine and panthenol compound preparation.