血卟啉单甲醚在银屑病免疫缺陷小鼠模型的体内分布

The distribution of HMME in psoriasis SCID mice model

目的观察血卟啉单甲醚(HMME)在银屑病免疫缺陷小鼠模型的体内分布情况,为光动力疗法治疗银屑病的机制提供科学依据。方法建立银屑病小鼠模型,尾静脉注射HMME10mg/kg,荧光分光光度法测定其在小鼠体内各脏器的分布,并比较HMME在银屑病皮损中与不同组织中的分布比。结果小鼠静脉注射HMME后,迅速分布于除脑外的其它各组织,注射1～3h后各组织药物浓度分别达峰值,其中以肝脏含量最高。给药后3h,银屑病皮损与正常皮肤内的药物分布比差距最大。于注射后24～48h,除肝脏和银屑病皮损尚存少量药物外,其余各组织未检测到药物存在。银屑病皮损中药物清除速度最慢。结论 HMME在银屑病免疫缺陷小鼠模型体内正常组织分布与代谢速度较快,正常皮肤的光敏风险较低;HMME不能透过小鼠血-脑屏障;给药后3h,HMME在银屑病皮损与正常皮肤中分布比差距最大,此时做光动力诊治可能效果更好。

Objective To objective the body distribution of HMME in SCID mice model,for investigate its clinical application perspective in psoriasis.Methods After the SCID mice model being established,thirty mice were administered intravenously with the HMME(10mg/kg).Fluorospectrophotometry was applied to measure the tissue distribution of HMME in SCID psoriasis mice model.Results The results showed that HMME distributed in mice immediately after intravenous administration.The maximal levels of photosensitizer concentration in each tissue were achieved at 1~3h post-injection,and the photosensitizer concentration of the liver was the highest among them.At 3h post-injection,the photosensitizer concentrations ratio of psoriasis lesion and skin was maximum.At 24~48h post-injection,the photosensitizer in other tissues had all been cleared except liver and psoriasis lesion remained only lower concentration of photosensitizer.And the clearance speed of photosensitizer in psoriasis lesion was the slowest.Conclusion The clearance speed of the normal tissue of HMME in SCID psoriasis mice model was fast,so the risk of skin's photosensitivity would not be high.HMME didn't diffuse through the blood-brain barrier.At 3h post-injection,the HMME concentrations ratio of psoriasis lesion and skin was maximum,which was the best time to PDT.